Journal ArticleDOI
Isolation and characterization of a glycosphingolipid having a new sialic acid.
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This article is published in Biochemistry.The article was published on 1969-12-01. It has received 88 citations till now. The article focuses on the topics: Glycosphingolipid & Sialic acid.read more
Citations
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Book ChapterDOI
Chemistry, Metabolism, and Biological Functions of Sialic Acids
TL;DR: This chapter discusses the chemistry, metabolism, and biological functions of sialic acids and the biosynthesis of N-acetylneuraminic acid is briefly reported and more attention is given to the enzyme reactions modifying this compound.
Journal ArticleDOI
Quantitative isolation of total glycosphingolipids from animal cells
TL;DR: The quantitative isolation of total glycosphingolipids from crude lipid extracts without contamination from other lipid classes is described and is useful for determination of microgram quantities of glycolipids derived from less than 1 ml of packed cells.
Journal ArticleDOI
Gas–liquid chromatographic assay of lipid-bound sialic acids: measurement of gangliosides in brain of several species
Robert K. Yu,Robert W. Ledeen +1 more
TL;DR: The GLC method has been applied to analysis of the total brain ganglioside content of several species, and a general trend was observed toward decreasing levels in the lower vertebrates.
Journal ArticleDOI
Ganglioside structures and distribution: Are they localized at the nerve ending?
TL;DR: Calculations based on reported values for axon terminal density and synaptosomal ganglioside concentration in the rat reveal that nerve endings contribute less than 12% of total cerebral cortical gang lioside, and other considerations suggest the possibility thatgangliosides may be distributed over the entire neuronal surface.
Journal ArticleDOI
Fluorometric high-performance liquid chromatography of N-acetyl- and N-glycolylneuraminic acids and its application to their microdetermination in human and animal sera, glycoproteins, and glycolipids.
TL;DR: A simple, rapid, and highly sensitive high-performance liquid chromatographic method is described for the determination of N-acetyl- and N-glycolylneuraminic acids in human and animal sera, glycoproteins, and glycolipids.
References
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Journal ArticleDOI
The thiobarbituric acid assay of sialic acids.
TL;DR: This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids.
Journal ArticleDOI
The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes
TL;DR: The effects of the ionic strength and pH of the hemolyzing solution on the hemoglobin content of human erythrocyte ghosts were studied in phosphate buffers and suggest an electrophysical interaction of hemoglobin with membrane constituents.
Journal ArticleDOI
Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method.
TL;DR: A new method for the quantitive determination of sialic acids is described, which is about 50% more sensitive than the orcinol-hydrochloric acid method generally used and considerably lower with the resorcin reagent.
Journal ArticleDOI