Open Access
Isolation and culture of human trabecular meshwork cells by extracellular matrix digestion
W. D. Stamer,Richard E. B. Seftor,Stuart K. Williams,H. A. M. Samaha,Robert W. Snyder +4 more
- Vol. 2, Iss: 49, pp 140
TLDR
In this article, an extracellular matrix digestion technique was used for the isolation of microvascular endothelial cells to isolate human trabecular meshwork cells, which was shown to be efficient and rapid for isolating large numbers of trabellular meshworks.Abstract:
Like corneal endothelial cells, human trabecular meshwork cells are believed to be of neural crest origin, but demonstrate physiological properties and an antithrombogenic surface similar to vascular endothelial cells. One current method for isolating trabecular meshwork cells utilizes the motile nature of these cells to migrate away from a trabecular meshwork explant in culture to more distal regions of the culture dish. This 'outgrowth' technique is limited in practice by the relatively small number of cells that migrate per explant per unit time, thus hindering the ability to gather sufficient numbers of cells for comprehensive experimentation. For this reason, we have modified an extracellular matrix digestion technique in current use for the isolation of microvascular endothelial cells to isolate human trabecular meshwork cells. This procedure is both efficient and rapid for isolating large numbers of trabecular meshwork cells and results in the availability of trabecular meshwork cells in sufficient quantities for subsequent experimentation.read more
Citations
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Journal ArticleDOI
TGFβ2-Induced Changes in Human Trabecular Meshwork: Implications for Intraocular Pressure
Debra L. Fleenor,Allan R. Shepard,Peggy E. Hellberg,Nasreen Jacobson,Iok-Hou Pang,Abbot F. Clark +5 more
TL;DR: Investigation of effects of TGFbeta2 on secretion of fibronectin and the protease inhibitor plasminogen activator inhibitor (PAI)-1 from human TM cell cultures and perfused human ocular anterior segments found modulation of T GFbeta2-induced changes in the ECM may provide a novel and viable approach to the management of glaucoma.
Journal ArticleDOI
Consensus recommendations for trabecular meshwork cell isolation, characterization and culture
Kate E. Keller,Sanjoy K. Bhattacharya,Theresa Borrás,Thomas M. Brunner,Sunee Chansangpetch,Abbott F. Clark,W. Michael Dismuke,Yiqin Du,Michael H. Elliott,C. Ross Ethier,Jennifer A. Faralli,Thomas F. Freddo,Rudolf Fuchshofer,Michael Giovingo,Haiyan Gong,Pedro Gonzalez,Alex S. Huang,Murray A. Johnstone,Paul L. Kaufman,Mary J. Kelley,Paul A. Knepper,Casey Kopczynski,John Kuchtey,Rachel W. Kuchtey,Markus H. Kuehn,Raquel L. Lieberman,Shan C. Lin,Paloma B. Liton,Yutao Liu,Elke Lütjen-Drecoll,Weiming Mao,Marisse Masis-Solano,Fiona McDonnell,Colleen M McDowell,Darryl R. Overby,Padmanabhan P. Pattabiraman,Vijay Krishna Raghunathan,P. Vasanth Rao,Douglas J Rhee,Uttio Roy Chowdhury,Paul Russell,John R. Samples,Donald Schwartz,Evan B. Stubbs,Ernst R. Tamm,James C. Tan,Carol B. Toris,Karen Y. Torrejon,Janice A. Vranka,Mary K. Wirtz,Thomas Yorio,Jie Zhang,Gulab Zode,Michael P. Fautsch,Donna M. Peters,Ted S. Acott,W. Daniel Stamer +56 more
TL;DR: Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.
Journal ArticleDOI
The many faces of the trabecular meshwork cell.
W. Daniel Stamer,Abbot F. Clark +1 more
TL;DR: The many responsibilities of the TM cell and its challenges are highlighted, progress in the understanding of TM biology over the past 30 years, as well as questions about TM dysfunction that results in IOP dysregulation and glaucoma are discussed.
Journal Article
Glucocorticoid induction of the glaucoma gene MYOC in human and monkey trabecular meshwork cells and tissues.
Abbot F. Clark,H. Thomas Steely,Jaime E. Dickerson,Sherry English-Wright,Karen Stropki,Mitchell D. McCartney,Nasreen Jacobson,Allan R. Shepard,John I. Clark,Hiroyuki Matsushima,Elaine R. Peskind,James B. Leverenz,Charles W. Wilkinson,Ruth E. Swiderski,John H. Fingert,Val C. Sheffield,Edwin M. Stone +16 more
TL;DR: Although the function of myocilin is unknown, induction of these TM proteins was found in eyes in which glucocorticoid-induced ocular hypertension developed, suggesting that myOCilin may play an important pathogenic role inOcular hypertension in addition to its role in certain forms of POAG.
Journal Article
Role of miR-29b on the regulation of the extracellular matrix in human trabecular meshwork cells under chronic oxidative stress.
TL;DR: The balance between the activation of ECM production induced by oxidative stress and the protective effects of miR-29b could be a relevant factor in understanding how oxidative damage may lead to increased deposition ofECM in the TM and contribute to the elevation of intra-ocular pressure in glaucoma.
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