Showing papers in "Experimental Eye Research in 2018"

TL;DR: 3D bioprinting is established to be a feasible method by which artificial corneal structures can be engineered and exhibited high cell viability both at day 1 post‐printing and at day 7.

TL;DR: Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.

TL;DR: The found the hub genes SLC17A7, NTRK2, ABTB1 and ADPRHL1 might play a vital role in recurrence of uveal melanoma, suggesting its critical role in the recurrence and development of the disease.

TL;DR: Narrow‐band, long‐wavelength lighting prevents the axial elongation typically produced by either form deprivation or hyperopicdefocus, possibly by creating direction signals normally associated with myopic defocus.

TL;DR: The possibility that HGF may represent a therapeutic tool for interrupting dysregulated corneal repair processes to improve patient outcomes is suggested.

TL;DR: The data show that there are various conspicuous features of foveolar Müller cells which may smooth the inner surface of the foveola, create additional barriers to the vitreous cavity, and provide mechanical stability to the tissue.

TL;DR: Only by working together (academia, industry and authorities) and by exploring parallel strategies (new drug delivery devices, enhanced drug formulations, better understanding of the pharmacokinetic properties), the therapeutic effect of drug treatments can be improved.

TL;DR: It is demonstrated that dry eye disease is accompanied by alterations in intraepithelial sensory nerve morphology and function and by reduced expression in corneal epithelial cells of mRNAs encoding genes mediating axon extension.

TL;DR: A comprehensive study of the time course of PR degeneration from the onset to one year of age, retinal structure by light and electron microscopy (EM), hemispheric asymmetry and gradients of rod and cone degeneration, rhodopsin content, gene dosage effect, rapid activation and invasion of the outer retina by presumptive microglia, rod outer segment disc shedding and phagocytosis by the retinal pigmented epithelium.

TL;DR: Bone marrow‐derived fibrocytes migrated to the cornea as early as 1 day after injury, and most GFP+ CD45+ &agr;SMA+ myofibroblasts that did develop subsequently underwent apoptosis—likely due to epithelial basement membrane regeneration and deprivation of epithelium‐derived TGF&bgr; requisite for my ofibroblast survival.

TL;DR: The results show that SARM1 is critical for RGC axonal degeneration and that axons rescued by S ARM1 deficiency are electrophysiologically active and suggest that somal activation of the DLK/JNK pathway is activated by an as‐yet‐unidentified SARM 1‐independent signal.

TL;DR: The bIOP correction has been successful in providing close estimates of true IOP in ex‐vivo tests conducted on human donor eye globes, and in reducing association with the cornea's thickness.

TL;DR: BNIP3L is identified as a novel requirement for the elimination of mitochondria, endoplasmic reticulum and Golgi apparatus during lens fiber cell remodeling and its co‐localization with lens organelles contributes toward organelle free zone formation in the developing lens.

TL;DR: A greater understanding of the mechanobiology of the corneal epithelium has the potential to lead to improved tissue engineering and cell based therapies to repair and regenerate damaged cornea.

TL;DR: A role is suggested for MiRNAs shuttled by extracellular vesicles as potential biomarkers of the onset of diabetic retinopathy as well as circulating EVs show differences between diabetic patients and healthy subjects.

TL;DR: The findings show that short‐term repeated GO exposure can cause obvious intraocular inflammation, an incrassated corneal stromal layer, cell apoptosis in the cornea, iris neovascularization and significant cytotoxicity of rat cornean epithelial cells, while RGO causes no significant ocular toxicity in mice.

TL;DR: In this paper, the authors used CRISPR/Cas9 technology to introduce protein-truncating germline lesions into the zebrafish ush2a gene (ush2armc1: c.2337_2342delinsAC; p.Cys780GlnfsTer32 and ush 2ab1245:c.15520_15523delinsTG, p.Ala5174fsTer). Homozygous mutants were viable and displayed no obvious morphological or developmental defects.

TL;DR: Serial section block‐face scanning electron microscopy for retinal pigment epithelium (SBFSEM) reveals that adult human RPE has hundreds of L, LF, and M and that granule spacing is regulated by granule size alone.

TL;DR: Exposure of pigmented mice to 3 consecutive days of blue LED light will cause RPE and photoreceptor damage, and this blue light exposed model may be useful for investigating the pathogenesis of non‐exudative age‐related macular degeneration.

TL;DR: This study has provided an unbiased glimpse into the transcriptome of three essential anterior ocular tissues, resulting in the development of several novel hypotheses and can be used in the future to better guide ocular research questions.

TL;DR: A non‐proliferative microglial reactivation was detected both in contralateral eyes and in OHT eyes in an early time after unilateral laser‐induced OHT (24 h), suggesting that this fast microglia activation, which involves thecontralateral eye, could be mediated by the immune system.

TL;DR: In albino mice, axotomy‐induced RGC death precedes the loss of their intraretinal axons and occurs in two phases, a rapid and a slower, but steady, one.

TL;DR: The data suggests a novel interaction between Nrf2 and ATF4 under oxidative and ER stress, thus drives specific enzymatic and non‐enzymatic reactions of antioxidant mechanisms maintaining redox homeostasis.

TL;DR: It is shown that human fetal retinal sheets transplanted to the severely disturbed subretinal space of RD nude rats develop mature photoreceptors and other retinal cells, integrate with the host and induce vision improvement.

TL;DR: H2S supplement effectively attenuated the apoptosis of RGC in experimental glaucoma, and the neuroprotection by H2S might correlate with preservation of mitochondrial function, attenuation of oxidative stress, suppression of glial activation, inhibition of inflammatory pathways and downregulation of autophagy.

TL;DR: In this paper, the authors correlated the hypo- and hyper-reflective bands on spectral domain optical coherence tomography (SD-OCT) images with histology of the lamellar architecture and cellular constituents of the porcine retina.

TL;DR: The present RNA‐seq data serves as a comprehensive reference resource for deciphering molecular principles of normal mammalian lens differentiation, mapping a full spectrum of signaling pathways and DNA‐binding transcription factors operating in both lens compartments, and predicting novel pathways required to establish lens transparency.

TL;DR: Investigation of crystallin isomerization as a function of age, location, protein solubility and tertiary structure concludes that the high Asp content of &agr;A is a hazard in terms of isomersization and aging, but it serves to enhance the refractive index of &ags;A relative to &agre;B, and may explain why &agR;B is only found in the eye.

TL;DR: The results from this experiment illustrate differences in ONH neural rim tissue, RNFL and vessel density changes with acute IOP challenge and support the hypothesis neural rim compression may be an important part of IOP‐induced damage.

TL;DR: The aim was to quantitatively compare retinal vascular detail as seen on optical coherence tomography angiography (OCTA) and matched histology in the human eye and to directly compare OCTA against histology from the same human eye.