Journal ArticleDOI
Isolation and partial characterization of bean phytohemagglutinins
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TLDR
In this paper, extracts of 21 bean cultivars were screened for hemagglutinating specifity and mitogenic activity and four types of mitogens were distinguished in different beans, two of which are mitogens.About:
This article is published in Phytochemistry.The article was published on 1974-12-01. It has received 52 citations till now. The article focuses on the topics: Phytohemagglutinins & Lectin.read more
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Book ChapterDOI
The lectins : carbohydrate-binding proteins of plants and animals
TL;DR: This chapter considers only those lectins that have been purified to homogeneity, and studied with regard to their biophysical, biochemical, and carbohydrate-binding specificity.
Journal ArticleDOI
Anti-nutritional and toxic factors in food legumes: a review
TL;DR: These factors are shown to be widely present in leguminous foods which are important constituents of the diet of a large section of the world's population, and particularly, of people in the developing countries.
Journal ArticleDOI
The Golgi apparatus mediates the transport of phytohemagglutinin to the protein bodies in bean cotyledons.
TL;DR: These results provide the first biochemical evidence that a specific storage protein which accumulates in seeds is modified in, and passes through, the Golgi apparatus on its way to the protein bodies.
Journal ArticleDOI
Composition and functional properties of Lupinus campestris protein isolates.
TL;DR: It is proven that some of the functional properties of L. campestrisprotein isolates are similar to those soybean protein isolates recovered under equal conditions, and the electrophoretic pattern of both protein isolate showed three bands with different mobilities, suggesting that the protein fractions belong to α-conglutin, β-congLutin and γ-congleutin.
Journal ArticleDOI
Biosynthesis, processing and transport of storage proteins and lectins in cotyledons of developing legume seeds
TL;DR: The storage proteins and lectins that accumulate in the protein bodies of developing legume cotyledons undergo a number of processing steps along the transport pathway from their site of synthesis to their site-of- deposition.
References
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Journal ArticleDOI
Lectins: cell-agglutinating and sugar-specific proteins.
Nathan Sharon,Halina Lis +1 more
Journal ArticleDOI
Separation of lymphocytes, polymorphonuclear leukocytes and monocytes on glass columns, including tissue culture observations.
Yale Rabinowitz,Yale Rabinowitz +1 more
TL;DR: The viability of column-separated cells was shown by their non-staining with trypan blue, motility, phagocytic ability, oxygen consumption, and survival or development in tissue culture, whereas Nowell’s blast-like, dividing, phytohemagglutinin cells were produced only in cultures containing lymphocytes.
Journal ArticleDOI
The structure of a phytohemagglutinin receptor site from human erythrocytes.
TL;DR: A highly purified glycopeptide with potent phytohemagglutinin (PHA) receptor site activity has been isolated from human erythrocyte membranes, showing that the inner core sugars can influence PHA-inhibitory activity.
Journal ArticleDOI
Phytohemagglutinin: inhibition of the agglutinating activity by N-acetyl-D-galactosamine.
H. Borberg,Judith J. Woodruff,Rochelle Hirschhorn,Bertram M. Gesner,P. Miescher,Robert Silber +5 more
TL;DR: N-Acetyl-d-galactosamine selectively inhibits the agglutination of leukocytes and erythro- cytes by phytohemagglutinin.
Journal ArticleDOI
Fractionation of proteins from kidney beans (phaseolus vulgaris).
TL;DR: Proteins extracted from the seeds of black kidney beans have been separated by ammonium sulfate fractionation and free-flow electrophoresis into fractions, but only two different hemagglutinating proteins could be demonstrated with reasonable certainty.