Journal ArticleDOI
K562--a human erythroleukemic cell line.
TLDR
The K562 cell expressed the major red cell sialoglycoprotein, glycophorin, on its surface as shown by immuno‐fluorescence and by immuneprecipitation from labelled membrane preparations, and it is concluded that the K562 is a human erythroleukemic line.Abstract:
We have studied the surface membrane properties of the human leukemic cell line K562 which previously has been reported to represent an early stage of granulocyte maturation. The surface glycoprotein pattern of the K562 cells obtained after galactose oxidase-NaB[3H]4 labelling and slab gel electrophoresis shows striking similarities with that of normal erythrocytes but is completely different from the patterns of normal and malignant cells of various stages of the myeloblast to granulocyte differentiation. Moreover, the K562 cell expressed the major red cell sialoglycoprotein, glycophorin, on its surface as shown by immunofluorescence and by immunoprecipitation from labelled membrane preparations. As glycophorin is exclusively found on erythroid cells in human bone marrow we conclude that the K562 is a human erythroleukemic line.read more
Citations
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Gold Nanoparticles Are Taken Up by Human Cells but Do Not Cause Acute Cytotoxicity
Journal ArticleDOI
Identification and characterization of essential genes in the human genome
Timothy C. Wang,Kıvanç Birsoy,Nicholas W. Hughes,Kevin M. Krupczak,Yorick Post,Yorick Post,Jenny J. Wei,Eric S. Lander,Eric S. Lander,Eric S. Lander,David M. Sabatini +10 more
TL;DR: Using the bacterial clustered regularly interspaced short palindromic repeats (CRISPR) system, this article constructed a genome-wide single-guide RNA library to screen for genes required for proliferation and survival in a human cancer cell line.
Identification and characterization of essential genes in the human genome
Timothy C. Wang,Kıvanç Birsoy,Nicholas W. Hughes,Kevin M. Krupczak,Yorick Post,Yorick Post,Jenny J. Wei,Eric S. Lander,Eric S. Lander,Eric S. Lander,David M. Sabatini +10 more
TL;DR: A genome-wide single-guide RNA library is constructed to screen for genes required for proliferation and survival in a human cancer cell line and reveals a set of cell-essential genes, which was validated with an orthogonal gene-trap–based screen and comparison with yeast gene knockouts.
Journal ArticleDOI
K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin.
TL;DR: The capacity of these cells to differentiate in vitro is investigated and it is reported here that they are induced by haemin to synthesise large amounts of haemoglobin; preliminary analysis indicates that this is predominantly of the embryonic type.
Journal ArticleDOI
The Approaching Era of the Tumor Suppressor Genes
TL;DR: Tumor suppressor genes will probably turn out to be as, if not more, diversified as the oncogenes and consideration of both kinds of genes may reveal common or interrelated functional properties.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels
TL;DR: A simple method for detecting 3H in polyacrylamide gels by scintillation autography (fluorography) using X-ray film, which is ten times more sensitive than conventional autoradiography of isotopes with higher emission energies.
Journal ArticleDOI
Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.
TL;DR: This CML cell-line represents a unique source of CML cells with meaningful indicators of malignancy for clinical and experimental studies.
Human chronic myelogenous leukemia cell-line with positive Philadelphia
CB Lozzio,BB Lozzio +1 more
TL;DR: This CML cell-line represents a unique source of CML cells with meaningful indicators of malignancy for clinical and experimental studies.