Kinetic studies of human immunodeficiency virus type 1 protease and its active-site hydrogen bond mutant A28S.
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TLDR
The lowering of pK values in mutant HIV-1 protease indicates that the hydroxyl group of Ser28 forms a new hydrogen bond to active-site Asp25 to increase its acidity.About:
This article is published in Journal of Biological Chemistry.The article was published on 1991-12-25 and is currently open access. It has received 141 citations till now. The article focuses on the topics: Active site & Protease.read more
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Journal ArticleDOI
Very fast empirical prediction and rationalization of protein pKa values
TL;DR: A very fast empirical method is presented for structure‐based protein pKa prediction and rationalization and unusual pKa values at buried active sites are predicted very well with the empirical method.
Journal ArticleDOI
Combining Conformational Flexibility and Continuum Electrostatics for Calculating pKas in Proteins
TL;DR: Multiconformation continuum electrostatics titrations show coupling of conformational flexibility and changes in ionization state and results are found with crystal and solution structures, showing that the method's explicit conformational sampling reduces sensitivity to the initial structure.
Journal ArticleDOI
The p2 domain of human immunodeficiency virus type 1 Gag regulates sequential proteolytic processing and is required to produce fully infectious virions.
Steve C. Pettit,Mark D. Moody,Robert S. Wehbie,Andrew H. Kaplan,Andrew H. Kaplan,Pascale V. Nantermet,Pascale V. Nantermet,C. Klein,Ronald Swanstrom +8 more
TL;DR: The findings suggest that the p2 domain of HIV-1 Gag regulates the rate of cleavage at the CA/p2 processing site during sequential processing in vitro and in infected cells and that p2 may function in the proper assembly of virions.
Journal ArticleDOI
Free energy calculations on dimer stability of the HIV protease using molecular dynamics and a continuum solvent model.
Wei Wang,Peter A. Kollman +1 more
TL;DR: The dominant negative inhibition of the HIV PR by a mutated form of the protease is examined and relative dimerization free energies of homo- and hetero-dimerization consistent with experimental data are found.
Journal ArticleDOI
Synthesis and virucidal activity of a water-soluble, configurationally stable, derivatized C60 fullerene.
TL;DR: The bis(monosuccinimide) derivative of p,p'-bis(2-aminoethyl)diphenyl-C60 (compound 1) is active against human immunodeficiency virus type 1 and HIV-2 in acutely or chronically infected human lymphocytes and is active in vitro against 3'-azido-3'-deoxythymidine-resistant HIV-1 (EC50, approximately 3 microM).
References
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Book ChapterDOI
Use of T7 RNA polymerase to direct expression of cloned genes.
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A pharmacokinetic analysis program (multi) for microcomputer.
TL;DR: A nonlinear least squares program (MULTI) for microcomputers was developed and the executions of MULTI are demonstrated for time courses of ampicillin and oxacillin in man.
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Complete nucleotide sequence of the AIDS virus, HTLV-III
Lee Ratner,William A. Haseltine,Roberto Patarca,Kenneth J. Livak,Bruno Starcich,Steven F. Josephs,Ellen R. Doran,J. Antoni Rafalski,Erik A. Whitehorn,Kirk Baumeister,Lucinda A. Ivanoff,Stephen R. Petteway,Mark L. Pearson,James A. Lautenberger,Takis S. Papas,John Ghrayeb,Nancy T. Chang,Robert C. Gallo,Flossie Wong-Staal +18 more
TL;DR: The complete nucleotide sequence of two human T-cell leukaemia type III (HTLV-III) proviral DNAs each have four long open reading frames, the first two corresponding to the gag and pol genes.
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Codon usage and tRNA content in unicellular and multicellular organisms.
TL;DR: Examination of silent substitutions and tRNA populations in Enterobacteriaceae revealed that the evolutionary constraint imposed by tRNA content on codon usage decelerated rather than accelerated the silent-substitution rate, at least insofar as pairs of taxonomically related organisms were examined.