Journal ArticleDOI
Kinetics and location of poliovirus macromolecular synthesis in correlation to virus-induced cytopathology
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TLDR
Viral protein synthesis is found to be at a maximum before virus-induced host cell alterations can yet be detected, and viral RNA synthesis, but not protein synthesis, is structurally tightly connected with the onset and increase of cytopathology.About:
This article is published in Virology.The article was published on 1980-01-30. It has received 87 citations till now. The article focuses on the topics: Poliovirus & Protein biosynthesis.read more
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Expression of Hepatitis C Virus Proteins Induces Distinct Membrane Alterations Including a Candidate Viral Replication Complex
Denise Egger,Benno Wölk,Rainer Gosert,Rainer Gosert,Leonardo Bianchi,Hubert E. Blum,Darius Moradpour,Kurt Bienz +7 more
TL;DR: It is proposed that the membranous web forms the viral replication complex in HCV-infected cells.
Journal ArticleDOI
Identification of the Hepatitis C Virus RNA Replication Complex in Huh-7 Cells Harboring Subgenomic Replicons
Rainer Gosert,Denise Egger,Volker Lohmann,Ralf Bartenschlager,Hubert E. Blum,Kurt Bienz,Darius Moradpour +6 more
TL;DR: Metabolic labeling with 5-bromouridine 5′-triphosphate in the presence of actinomycin D revealed that the membranous web is the site of viral RNA synthesis and therefore represents the replication complex of HCV.
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Association of polioviral proteins of the P2 genomic region with the viral replication complex and virus-induced membrane synthesis as visualized by electron microscopic immunocytochemistry and autoradiography.
TL;DR: Using high resolution electron microscopic autoradiography and immunocytochemistry with monoclonal antibodies against poliovirus proteins of the P2 genomic region, the location of these proteins in respect to the virus-induced vesicle formation and the viral RNA synthesis was followed during the viral replication cycle.
Journal ArticleDOI
Structural and functional characterization of the poliovirus replication complex.
TL;DR: It was found that the progeny RNA strands are set free soon after completion from the replication complex at the sites where the compact membranes within the replicationcomplex are in close contact with the surrounding virus-induced vesicles.
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Structural organization of poliovirus RNA replication is mediated by viral proteins of the P2 genomic region.
TL;DR: The findings allow the conclusion that the protein 2C or 2C-containing precursor(s) is responsible for the attachment of the viral RNA to the vesicular membrane and for the spatial organization of the replication complex necessary for its proper functioning in viral transcription.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels
TL;DR: A simple method for detecting 3H in polyacrylamide gels by scintillation autography (fluorography) using X-ray film, which is ten times more sensitive than conventional autoradiography of isotopes with higher emission energies.
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Polypeptide cleavages in the formation of poliovirus proteins
TL;DR: It is found that cleavages play an important role in the formation of most if not all poliovirus-specific proteins.