Journal ArticleDOI
Label-free fluorescent assay of T4 polynucleotide kinase phosphatase activity based on G-quadruplexe-thioflavin T complex.
Reads0
Chats0
TLDR
This work proposed a novel label-free sensing strategy for T4 PNKP activity based on G-quadruplexe-thioflavin T fluorescent indicator that is easy and convenient to operate in homogeneous solution, and the whole assay process can be completed in a single tube.About:
This article is published in Talanta.The article was published on 2017-04-01. It has received 25 citations till now.read more
Citations
More filters
Journal ArticleDOI
Thioflavin T as a fluorescence probe for biosensing applications
TL;DR: Thioflavin T, a water-soluble fluorescence probe and the conventional dye for the detection of amyloid fibrils has recently been demonstrated to recognize and bind nucleic acids and helps in the formation of G-quadruplex (G4) structure.
Journal ArticleDOI
Label-Free G-Quadruplex Aptamer Fluorescence Assay for Ochratoxin A Using a Thioflavin T Probe.
TL;DR: This method has a high specificity for OTA relative to Ochratoxin B (23%) and Aflatoxin B1 (13%) and has been validated using a sample of red wine supplemented with a range of OTA concentrations with recoveries of 96.5% to 107%.
Journal ArticleDOI
Ultrasensitive, label-free detection of T4 ligase and T4 polynucleotide kinase based on target-triggered hyper-branched rolling circle amplification
TL;DR: A simple, convenient and ultrasensitive fluorescence biosensor for label-free detection of ligase and PNK was proposed on the basis of target-triggered hyper-branched rolling circle amplification (HRCA) to offer a promising tool in clinical molecular diagnostics and cancer therapy.
Journal ArticleDOI
Label-free and nicking enzyme-assisted fluorescence signal amplification for RNase H determination based on a G-quadruplexe/thioflavin T complex.
TL;DR: A novel, label-free and nicking enzyme-assisted fluorescence signal amplification strategy that demonstrates to be cost efficient, sensitive, and unique for assaying the RNase H activity and inhibition based on G-quadruplex formation using a thioflavin T (ThT) dye is described.
Journal ArticleDOI
Dimeric G-Quadruplex: An Effective Nucleic Acid Scaffold for Lighting Up Thioflavin T.
TL;DR: It is demonstrated that dimeric G4 is an effective nucleic acid scaffold for lighting up ThT, showing promising applications in a label-free bioassay.
References
More filters
Journal ArticleDOI
The causes and prevention of cancer.
TL;DR: Epidemiological evidence indicates that avoidance of smoking, increased consumption of fruits and vegetables, and control of infections will have a major effect on reducing rates of cancer.
Journal ArticleDOI
Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli
TL;DR: The activating effects of PLP and Fe+++ on these earlier preparations remain unexplained but were partially due to stabilizing effects on the enzyme under the assay conditions then in use, and hence provide no evidence for a role ofPLP in action of the enzyme.
Journal ArticleDOI
Thioflavin T as an Efficient Inducer and Selective Fluorescent Sensor for the Human Telomeric G-Quadruplex DNA
Jyotirmayee Mohanty,Nilotpal Barooah,V. Dhamodharan,S. Harikrishna,Pushpangadan Indira Pradeepkumar,Achikanath C. Bhasikuttan +5 more
TL;DR: The application of a water-soluble fluorogenic dye, Thioflavin T (ThT), in a dual role of exclusively inducing quadruplex folding in the 22AG human telomeric DNA, both in the presence and absence of Tris buffer/salt, is demonstrated.
Journal ArticleDOI
Evolution of Water Lifting Devices (Pumps) over the Centuries Worldwide
Stavros Yannopoulos,Gerasimos Lyberatos,Nicolaos Theodossiou,Wang Li,Mohammad Valipour,Aldo Tamburrino,Andreas N. Angelakis +6 more
TL;DR: A timeline of the historical development of water pumps worldwide through the last 5500 years of the history of mankind is presented in this article, followed by a chronological order with emphasis on the major civilizations.
Journal ArticleDOI
Optimization of neural networks for precipitation analysis in a humid region to detect drought and wet year alarms
TL;DR: Performance of the networks was not satisfactory because the number of neurons in the hidden layer and the roles of training, validation and testing phases were lacking flexibility and change, but improvement of network accuracy was found.