Book ChapterDOI
Label-Free Immuno-Sensors for the Fast Detection of Listeria in Food.
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TLDR
An immune-sensor, based on Surface Plasmon Resonance imaging (SPRi) and bacterial growth which allows the detection of a very low number of Listeria monocytogenes in food sample in one day, and reduces handling and time to result compared with current methods.Abstract:
Foodborne diseases are a major concern for both food industry and health organizations due to the economic costs and potential threats for human lives. For these reasons, specific regulations impose the research of pathogenic bacteria in food products. Nevertheless, current methods, references and alternatives, take up to several days and require many handling steps. In order to improve pathogen detection in food, we developed an immune-sensor, based on Surface Plasmon Resonance imaging (SPRi) and bacterial growth which allows the detection of a very low number of Listeria monocytogenes in food sample in one day. Adequate sensitivity is achieved by the deposition of several antibodies in a micro-array format allowing real-time detection. This label-free method thus reduces handling and time to result compared with current methods.read more
Citations
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Journal ArticleDOI
Electrochemical and Optical Biosensors for the Detection of Campylobacter and Listeria: An Update Look.
TL;DR: It is expected that high-performance of devices being developed through basic research will find extensive applications in environmental monitoring, biomedical diagnostics, and food safety.
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Recent advancements in microfluidic chip biosensor detection of foodborne pathogenic bacteria: a review
TL;DR: In this article , the authors reviewed the bio-recognition elements integrated on microfluidic chips in recent years and the progress of micro-device development for pathogen pretreatment.
Journal ArticleDOI
Ultrasensitive and Fast Diagnostics of Viable Listeria Cells by CBD Magnetic Separation Combined with A511::luxAB Detection.
TL;DR: An ultrasensitive diagnostic protocol for Listeria is described by combining two phage-based strategies: specific capture and concentration of target cells by magnetic separation, and highly sensitive detection using an adaptation of the A511::luxAB bioluminescent reporter phage assay in a microwell plate format.
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A Label-Free and Anti-Interference Dual-Channel SPR Fiber Optic Sensor With Self-Compensation for Biomarker Detection
TL;DR: A small-in-size, simple-to-manufacture, dual-channel SPR sensor with self-compensation with an excellent application prospect in the clinical monitoring of various biomarkers is proposed and demonstrated.
Journal ArticleDOI
Immunosensors—The Future of Pathogen Real-Time Detection
E. Janik-Karpinska,Michał Ceremuga,Marcin Niemcewicz,Marcin Podogrocki,Maksymilian Stela,Natalia Cichon,Michal Bijak +6 more
TL;DR: In this article , the advances in the field of immunosensor usage in pathogenic microorganism- and toxin-detection, are described, and a very important potential use for them in various transport systems, where the risk of contamination by bioagents is very high.
References
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Journal ArticleDOI
Surveillance of listeriosis and its causative pathogen, Listeria monocytogenes
Ewen C. D. Todd,S. Notermans +1 more
TL;DR: In addition to outbreak investigation, case-control studies, and the use of experts, risk assessments, and food attribution studies can help focus on areas of greatest risk for prevention and control measures throughout the food chain.
Journal ArticleDOI
Rapid detection and identification methods for Listeria monocytogenes in the food chain – A review
TL;DR: A wide variety of culture and alternative methods have been developed in order to detect or quantify this pathogen in food, and here are presented the most rapid and sensitive methods.
Journal ArticleDOI
On-chip microbial culture for the specific detection of very low levels of bacteria
Sihem Bouguelia,Yoann Roupioz,Sami Slimani,Laure Mondani,Maria G Casabona,Claire Durmort,Claire Durmort,Thierry Vernet,Thierry Vernet,Roberto Calemczuk,Thierry Livache +10 more
TL;DR: In this paper, the authors combine concomitant "on-chip" microbial culture with sensitive surface plasmon resonance (SPR) detection of bacteria, thus allowing rapid specific detection of the bacteria pathogens.
Journal ArticleDOI
Real-time pathogen monitoring during enrichment: a novel nanotechnology-based approach to food safety testing
Kristin Weidemaier,Erin Carruthers,Adam C. Curry,Melody Kuroda,Eric Fallows,Joseph Thomas,Douglas B. Sherman,Mark Muldoon +7 more
TL;DR: Surface Enhanced Raman Scattering (SERS) nanoparticles are combined with a novel homogeneous immunoassay to allow sensitive detection of pathogens in complex samples such as stomached food without the need for wash steps or extensive sample preparation.
Book ChapterDOI
Bacteria: Listeria monocytogenes
R. Magalhães,Cristina Mena,Vânia Ferreira,Joana Silva,Gonçalo Almeida,Paul Gibbs,Paula Teixeira +6 more
TL;DR: This article will summarize what is currently known about L. monocytogenes, listeriosis, methods of detection and enumeration, subtyping, persistence, virulence traits, and regulatory control.