Journal ArticleDOI
Large platelets circulate in an activated state in diabetes mellitus
TLDR
It is concluded that in diabetes mellitus an increased number of large platelets circulate in an activated state predominantly in patients with angiopathy, which could imply that platelets become activated by vascular lesions.Abstract:
Diabetes mellitus is associated with aggravated development of vascular complications. Yet, it has not been established whether platelet hyperreactivity contributes as a pathogenetic factor. In order to study the role of activated platelets in diabetes mellitus, we investigated the expression of the membrane activation markers CD63 (GP53) and CD62 (GMP-140) as direct indicators of in vivo activation. The CD63-positive fraction was significantly higher in patients (6.1% X 3.7 +/- 1) than in controls (2.7% X 3 +/- 1). In parallel, the CD62-positive fraction was significantly elevated in patients to 5% X 2.5 +/- 1 in comparison to controls (3% X 2 +/- 1). Patients with angiopathy had a mean increase of 304% in CD63-positive and of 223% in CD62-positive platelets. Patients without clinically detectable angiopathy showed a trend to an increased fraction in CD63-/CD62-positive platelets. There was no correlation of the activation markers with fasting blood glucose, HbA1 or platelet count. CD63 platelet bound fluorescence significantly increased with platelet size in the patient group. We conclude that in diabetes mellitus an increased number of large platelets circulate in an activated state predominantly in patients with angiopathy. This could imply that platelets become activated by vascular lesions. The trend in patients without vascular disease, however, suggests that activated platelets may also basically contribute to the prethrombotic state in diabetes mellitus.read more
Citations
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Journal ArticleDOI
Platelet Function Profiles in Patients With Type 2 Diabetes and Coronary Artery Disease on Combined Aspirin and Clopidogrel Treatment
Dominick J. Angiolillo,Antonio Fernández-Ortiz,Esther Bernardo,Celia Ramírez,Manel Sabaté,Pilar Jiménez-Quevedo,Rosana Hernández,Raul Moreno,Javier Escaned,Fernando Alfonso,Camino Bañuelos,Marco A. Costa,Theodore A. Bass,Carlos Macaya +13 more
TL;DR: Diabetic patients have increased platelet reactivity compared with nondiabetic subjects on combined aspirin and clopidogrel treatment and reduced sensitivity to antiplatelet drugs may contribute to the increased atherothombotic risk in diabetic patients.
Journal ArticleDOI
Platelet activation: assessment and quantification
TL;DR: Despite the great success of aspirin, it is clearly far fromperfect that there is still no ideal measure of platelet activation that would indicate a state of ‘high risk’.
Journal ArticleDOI
European Working Group on Clinical Cell Analysis: Consensus Protocol for the Flow Cytometric Characterisation of Platelet Function
Gerd Schmitz,Gregor Rothe,A. Ruf,Stefan Barlage,D Tschöpe,Kenneth J. Clemetson,Alison H. Goodall,Alan D. Michelson,Alan T. Nurden,T V Shankey +9 more
TL;DR: Specific flow cytometry techniques for platelet analysis are recommended based on a description of the current state of flow cytometric methodology in an attempt to promote the use of these new techniques which are at present broadly evaluated for diagnostic purposes.
Journal ArticleDOI
The relationship of mean platelet volume with the risk and prognosis of cardiovascular diseases.
TL;DR: Mean platelet volume is arousing increasing interest as a new independent cardiovascular risk factor and may be a potential new independent risk factor for heart attack and stroke.
References
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Journal ArticleDOI
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Journal ArticleDOI
Detection of activated platelets in whole blood using activation- dependent monoclonal antibodies and flow cytometry
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Journal ArticleDOI
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