Light-based feedback for controlling intracellular signaling dynamics
TLDR
'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction is reported, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity.Abstract:
The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an 'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity.read more
Citations
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疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A
TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
Journal ArticleDOI
Encoding and decoding cellular information through signaling dynamics.
Jeremy E. Purvis,Galit Lahav +1 more
TL;DR: This Review discusses potential functional roles for transmitting cellular information through the dynamics of signaling molecules and possible applications for the treatment of disease.
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Spatiotemporal Control of Intracellular Phase Transitions Using Light-Activated optoDroplets
Yongdae Shin,Joel Berry,Nicole L. Pannucci,Mikko Haataja,Jared E. Toettcher,Clifford P. Brangwynne +5 more
TL;DR: An optogenetic platform that uses light to activate IDR-mediated phase transitions in living cells to elucidate not only physiological phase transitions but also their link to pathological aggregates is introduced.
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Remote control of therapeutic T cells through a small molecule-gated chimeric receptor
TL;DR: The ON-switch CAR exemplifies a simple and effective strategy to integrate cell-autonomous decision-making with exogenous, reversible user control and highlights the importance of developing optimized bio-inert, orthogonal control agents such as small molecules and light, together with their cellular cognate response components, in order to advance precision-controlled cellular therapeutics.
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Using Optogenetics to Interrogate the Dynamic Control of Signal Transmission by the Ras/Erk Module
TL;DR: An optogenetic approach to selectively activate isolated intracellular signaling nodes with light is developed and used to identify signaling programs that differentially respond to Ras dynamics, including a paracrine circuit that activates STAT3 only after persistent Ras activation.
References
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疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A
TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
Journal ArticleDOI
Enzymatic assembly of DNA molecules up to several hundred kilobases
Daniel G. Gibson,Lei Young,Ray-Yuan Chuang,J. Craig Venter,Clyde A. Hutchison,Hamilton O. Smith +5 more
TL;DR: An isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5′ exonuclease, a DNA polymerase and a DNA ligase is described.
Journal ArticleDOI
Computer control of microscopes using µManager.
Arthur D. Edelstein,Nenad Amodaj,Karl Hoover,Ronald D. Vale,Ronald D. Vale,Nico Stuurman,Nico Stuurman +6 more
TL;DR: This unit provides step‐by‐step protocols describing how to get started working with µManager, as well as some starting points for advanced use of the software.
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Measurement of current-voltage relations in the membrane of the giant axon of Loligo.
TL;DR: The importance of ionic movements in excitable tissues has been emphasized by a number of recent experiments which are consistent with the theory that nervous conduction depends on a specific increase in permeability which allows sodium ions to move from the more concentrated solution outside a nerve fibre to the more dilute solution inside it.
Journal ArticleDOI
A stable human-derived packaging cell line for production of high titer retrovirus/vesicular stomatitis virus G pseudotypes
TL;DR: A human 293-derived retroviral packaging cell line (293GPG) capable of producing high titers of recombinant Moloney murine leukemia virus particles that have incorporated the vesicular stomatitis virus G (VSV-G) protein is generated.