Liposome-Encapsulated Bacteriophages for Enhanced Oral Phage Therapy against Salmonella spp.
Joan Colom,Mary Cano-Sarabia,Jennifer Otero,Pilar Cortés,Daniel Maspoch,Montserrat Llagostera +5 more
TLDR
The methodology described here allows the liposome encapsulation of phages of different morphologies to be stored for at least 3 months at 4°C and could be added to the drinking water and feed of animals.Abstract:
Bacteriophages UAB_Phi20, UAB_Phi78, and UAB_Phi87 were encapsulated in liposomes, and their efficacy in reducing Salmonella in poultry was then studied. The encapsulated phages had a mean diameter of 309 to 326 nm and a positive charge between +31.6 and +35.1 mV (pH 6.1). In simulated gastric fluid (pH 2.8), the titer of nonencapsulated phages decreased by 5.7 to 7.8 log units, whereas encapsulated phages were significantly more stable, with losses of 3.7 to 5.4 log units. The liposome coating also improved the retention of bacteriophages in the chicken intestinal tract. When cocktails of the encapsulated and nonencapsulated phages were administered to broilers, after 72 h the encapsulated phages were detected in 38.1% of the animals, whereas the nonencapsulated phages were present in only 9.5%. The difference was significant. In addition, in an in vitro experiment, the cecal contents of broilers promoted the release of the phages from the liposomes. In broilers experimentally infected with Salmonella, the daily administration of the two cocktails for 6 days postinfection conferred similar levels of protection against Salmonella colonization. However, once treatment was stopped, protection by the nonencapsulated phages disappeared, whereas that provided by the encapsulated phages persisted for at least 1 week, showing the enhanced efficacy of the encapsulated phages in protecting poultry against Salmonella over time. The methodology described here allows the liposome encapsulation of phages of different morphologies. The preparations can be stored for at least 3 months at 4°C and could be added to the drinking water and feed of animals.read more
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Formulation, stabilisation and encapsulation of bacteriophage for phage therapy
Danish J. Malik,I. J. Sokolov,Gurinder K. Vinner,Francesco Mancuso,Salvatore Cinquerrui,Goran T. Vladisavljevic,Martha R. J. Clokie,Natalie J. Garton,Andrew G. F. Stapley,Anna Kirpichnikova +9 more
TL;DR: The clinical needs and challenges associated with treatment of acute and chronic infections and the drivers for phage encapsulation are looked at, as well as looking at promising new approaches for micro- and nanoencapsulation of phage and how these may address gaps in the field.
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Gram-Positive Bacterial Extracellular Vesicles and Their Impact on Health and Disease
TL;DR: The rapidly advancing research into Gram-positive EVs is currently in a crucial phase, therefore this review aims to give an overview of the groundwork that has been laid at present and to discuss implications and future challenges of this new research field.
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Phage therapy: What factors shape phage pharmacokinetics and bioavailability? Systematic and critical review.
TL;DR: This review provides a comprehensive overview of factors that determinephage circulation, penetration, and clearance, and that in consequence determine phage applicability for medicine.
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Bacteriophage Procurement for Therapeutic Purposes.
Beata Weber-Dąbrowska,Ewa Jończyk-Matysiak,Maciej Żaczek,Małgorzata Łobocka,Marzanna Łusiak-Szelachowska,Andrzej Górski +5 more
TL;DR: This article attempts to summarize the state of the art: properties of phages, the methods for their isolation, criteria of phage selection for therapeutic purposes and limitations of their use.
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Phage therapy for respiratory infections.
Rachel Yoon Kyung Chang,Martin Wallin,Yu Lin,Sharon Sui Yee Leung,Hui Wang,Sandra Morales,Hak-Kim Chan +6 more
TL;DR: Inhaled phage therapy has the potential to transform the prevention and treatment of bacterial respiratory infections, including those caused by antibiotic‐resistant bacteria.
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