MADS-Box Protein Complexes Control Carpel and Ovule Development in Arabidopsis
Rebecca Favaro,Anusak Pinyopich,Raffaella Battaglia,Maarten Kooiker,Lorenzo Borghi,Gary S. Ditta,Martin F. Yanofsky,Martin M. Kater,Lucia Colombo +8 more
TLDR
It is shown that ectopic expression of either the STK or SHP gene is sufficient to induce the transformation of sepals into carpeloid organs bearing ovules and that the SEP proteins, known to form multimeric complexes in the control of flower organ identity, also form complexes to control normal ovule development.Abstract:
The AGAMOUS (AG) gene is necessary for stamen and carpel development and is part of a monophyletic clade of MADS-box genes that also includes SHATTERPROOF1 (SHP1), SHP2, and SEEDSTICK (STK). Here, we show that ectopic expression of either the STK or SHP gene is sufficient to induce the transformation of sepals into carpeloid organs bearing ovules. Moreover, the fact that these organ transformations occur when the STK gene is expressed ectopically in ag mutants shows that STK can promote carpel development in the absence of AG activity. We also show that STK, AG, SHP1, and SHP2 can form multimeric complexes and that these interactions require the SEPALLATA (SEP) MADS-box proteins. We provide genetic evidence for this role of the SEP proteins by showing that a reduction in SEP activity leads to the loss of normal ovule development, similar to what occurs in stk shp1 shp2 triple mutants. Together, these results indicate that the SEP proteins, which are known to form multimeric complexes in the control of flower organ identity, also form complexes to control normal ovule development.read more
Citations
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Genetic regulation of fruit development and ripening
TL;DR: Light is shed on the molecular basis of developmental ripening control, suggested common regulators of climacteric and nonclimacteric ripening physiology, and how these regulators affect human and animal diets.
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The SEP4 Gene of Arabidopsis thaliana Functions in Floral Organ and Meristem Identity
TL;DR: The SEP4 gene is characterized, which shares extensive sequence similarity to and an overlapping expression pattern with the other SEP genes, and it is found that it contributes to the development of petals, stamens, and carpels in addition to sepals and that it plays an important role in meristem identity.
Journal ArticleDOI
Comprehensive Interaction Map of the Arabidopsis MADS Box Transcription Factors
Stefan de Folter,Richard G. H. Immink,Martin Kieffer,Lucie Pařenicová,Stefan R. Henz,Detlef Weigel,Marco Busscher,Maarten Kooiker,Lucia Colombo,Martin M. Kater,Brendan Davies,Gerco C. Angenent +11 more
TL;DR: A comprehensive plant protein–protein interactome map of nearly all members of the Arabidopsis thaliana MADS box transcription factor family is presented and a model is proposed that integrates the floral induction and floral organ formation networks based on the interactions between the proteins involved.
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Molecular Mechanisms of Flower Development: An Armchair Guide
TL;DR: The transcriptional regulatory cascades that control early patterning events during flower formation, the dynamics of the gene-regulatory interactions, and the complex combinatorial mechanisms that create a distinct final floral architecture and form are revealed.
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MIKC-type MADS-domain proteins: structural modularity, protein interactions and network evolution in land plants.
TL;DR: In this paper, the authors hypothesize that combinatorial multimer formation of MIKC-type MADS-domain proteins facilitated an unusually efficient and rapid functional diversification based on gene duplication, sequence divergence and fixation.
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