Journal ArticleDOI
Med8, a subunit of the mediator CTD complex of RNA polymerase II, directly binds to regulatory elements of SUC2 and HXK2 genes.
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TLDR
The partially purified p27 protein identified the MED8 gene (open reading frame YBR193C), located in chromosome II of S. cerevisiae, as the gene coding for the protein, suggesting that Med8 may be important for the coupling of the glucose repression pathway of SUC2 gene to the HXK2 gene expression.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1999-01-19. It has received 35 citations till now. The article focuses on the topics: Transcription factor II D & RNA polymerase II holoenzyme.read more
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Journal ArticleDOI
Transcription of eukaryotic protein-coding genes
Tong Ihn Lee,Richard A. Young +1 more
TL;DR: Major advances in knowledge of transcriptional regulation involve the chromatin template, the large complexes recruited by transcriptional activators that regulate chromatin structure and the transcription apparatus, the holoenzyme forms of RNA polymerase II involved in initiation and elongation, and the mechanisms that link mRNA processing with its synthesis.
Journal ArticleDOI
Glucose Signaling in Saccharomyces cerevisiae
TL;DR: Fine-structure mapping of nuclear architecture will be required to understand the reception of regulatory signals that emanate from the plasma membrane and cytoplasm, which should result in a much improved understanding of eukaryotic growth, differentiation, and carcinogenesis.
Journal ArticleDOI
Mediator of transcriptional regulation.
TL;DR: Three lines of evidence have converged on a multiprotein Mediator complex as a conserved interface between gene-specific regulatory proteins and the general transcription apparatus of eukaryotes, which functions directly through RNA polymerase II, modulating its activity in promoter-dependent transcription.
Journal ArticleDOI
The early steps of glucose signalling in yeast
TL;DR: It is shown that in Saccharomyces cerevisiae, sensing systems for other nutrients share some of the characteristics of the glucose-sensing pathways, as well as other regulatory elements whose functions are still incompletely understood.
Journal ArticleDOI
Moonlighting Proteins in Yeasts
TL;DR: This review considers several well-studied moonlighting proteins in different yeast species, paying attention to the experimental approaches used to identify them and the evidence that supports their participation in the unexpected function.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Basic Local Alignment Search Tool
TL;DR: A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score.
Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins
TL;DR: A 30-amino-acid segment of C/EBP, a newly discovered enhancer binding protein, shares notable sequence similarity with a segment of the cellular Myc transforming protein, and may represent a characteristic property of a new category of DNA binding proteins.
Journal ArticleDOI
Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertase
Marian Carlson,David Botstein +1 more
TL;DR: A model is proposed to account for the synthesis and regulation of the two forms of inverts: the larger, regulated mRNA contains the initiation codon for the signal sequence required for synthesis of the secreted, glycosylated form of invertase; the smaller, constitutively transcribed mRNA begins within the coding region of the signal sequences, resulting in synthesis ofThe intracellular enzyme.