Metabolic Engineering for Production of β-Carotene and Lycopene in Saccharomyces cerevisiae
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TLDR
A conventional yeast is engineered to confer a novel biosynthetic pathway for the production of beta-carotene and lycopene by introducing the bacterial carotenoid biosynthesis genes, which are individually surrounded by the promoters and terminators derived from S. cerevisiae.Abstract:
We have engineered a conventional yeast, Saccharomyces cerevisiae, to confer a novel biosynthetic pathway for the production of β-carotene and lycopene by introducing the bacterial carotenoid biosynthesis genes, which are individually surrounded by the promoters and terminators derived from S. cerevisiae. β-Carotene and lycopene accumulated in the cells of this yeast, which was considered to be a result of the carbon flow for the ergosterol biosynthetic pathway being partially directed to the pathway for the carotenoid production.read more
Citations
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Journal ArticleDOI
Terpenoids: opportunities for biosynthesis of natural product drugs using engineered microorganisms.
Parayil Kumaran Ajikumar,Keith E. J. Tyo,Simon Carlsen,Oliver Mucha,Too Heng Phon,Gregory Stephanopoulos +5 more
TL;DR: This review focuses on the biodiversity of terpenoids, the biosynthetic pathways involved, and engineering efforts to maximize the production through these pathways.
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Biotechnological production of carotenoids by yeasts: an overview.
TL;DR: The general and applied concepts regarding yeasts carotenoid production and the factors influencingCarotenogenesis using agro-industrial wastes as low-cost substrates are discussed.
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Improving industrial yeast strains: exploiting natural and artificial diversity
Jan Steensels,Tim Snoek,Esther Meersman,Martina Picca Nicolino,Karin Voordeckers,Kevin J. Verstrepen +5 more
TL;DR: The different strategies of strain selection and improvement available for both conventional and nonconventional yeasts are discussed, providing a new source of yeast genetic diversity.
Journal ArticleDOI
High-Level Production of Beta-Carotene in Saccharomyces cerevisiae by Successive Transformation with Carotenogenic Genes from Xanthophyllomyces dendrorhous
René Verwaal,Jing Wang,Jean-Paul Meijnen,Hans Visser,Gerhard Sandmann,Johan A. van den Berg,Albert J. J. van Ooyen +6 more
TL;DR: It is succeeded in constructing an S. cerevisiae strain capable of producing high levels of β-carotene, up to 5.9 mg/g (dry weight), which was accomplished by the introduction of an additional copy of crtI and tHMG1 into carotenoid-producing yeast cells.
Journal ArticleDOI
Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production.
Seon-Won Kim,Jay D. Keasling +1 more
TL;DR: Given the low final densities of cells expressing dxs from IPTG-inducible promoters, the low lycopene production was probably due to the metabolic burden of plasmid maintenance and an excessive drain of central metabolic intermediates.
References
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