Journal ArticleDOI
Methylation and cleavage sequences of the EcoP1 restriction-modification enzyme☆
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TLDR
The sites of both cleavage and methylation in simian virus 40 DNA are mapped and the enzyme EcoP1 methylation and cleavage site is determined.About:
This article is published in Journal of Molecular Biology.The article was published on 1979-02-25. It has received 84 citations till now. The article focuses on the topics: DNA methylation & Methylation.read more
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Biology of DNA restriction.
Thomas A. Bickle,D H Krüger +1 more
TL;DR: The discovery of a new class of restriction systems that specifically cut DNA carrying the modification signature of foreign cells, and the mechanisms developed by phages to avoid the restriction systems of their hosts are described.
Journal ArticleDOI
Characterization of DNA methyltransferase specificities using single-molecule, real-time DNA sequencing
Tyson A. Clark,Iain A. Murray,Richard D. Morgan,Andrey Kislyuk,Kristi E. Spittle,Matthew Boitano,Alexey Fomenkov,Richard J. Roberts,Jonas Korlach +8 more
TL;DR: SMRT sequencing confirms the identity and position of the methylated base in cases where the MTase specificity was previously established by other methods and finds evidence of unanticipated MTase promiscuity with some enzymes apparently also modifying sequences that are related, but not identical to the cognate site.
Journal ArticleDOI
Bacteriophage survival: multiple mechanisms for avoiding the deoxyribonucleic acid restriction systems of their hosts.
D H Krüger,Thomas A. Bickle +1 more
TL;DR: Les bacteriophages ont appris a vivre avec les enzymes de restriction de leur hote en developpant de nombreux mecanismes differents pour eviter les pires effets des systemes de restriction.
Journal ArticleDOI
The CRISPRs, They Are A-Changin': How Prokaryotes Generate Adaptive Immunity
Edze R. Westra,Daan C. Swarts,Raymond H.J. Staals,Matthijs M. Jore,Stan J. J. Brouns,John van der Oost +5 more
TL;DR: This review focuses on the type I-E CRISPR/Cas system of the model bacterium Escherichia coli K12, a prokaryotic adaptive immune system that displays major structural and functional differences in their mode of generating resistance against invading nucleic acids.
Journal ArticleDOI
Genome of Bacteriophage P1
Małgorzata Łobocka,Debra J. Rose,Guy Plunkett,Marek Rusin,Arkadiusz Samojedny,Hansjörg Lehnherr,Michael Yarmolinsky,Frederick R. Blattner +7 more
TL;DR: The base content and distribution in P1 DNA indicate that replication of P1 from its plasmid origin had more impact on the base compositional asymmetries of the P1 genome than replication from the lytic origin of replication.
References
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Journal ArticleDOI
Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
TL;DR: It is demonstrated that pre-exposure of the film to a brief flash of light greatly increases the sensitivity of fluorography, because it corrects the non-linear relationship between radioactivity of the sample and absorbance of theFilm image.
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A simple method for the preparation of 32P-labelled adenosine triphosphate of high specific activity
IM Glynn,JB Chappell +1 more
TL;DR: Hydrolysis of the labeled ATP with heavy meromyosin indicates that 98 to 99% of the /sup 32/P is in the 1 -phosphate group.
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Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis
Journal ArticleDOI
Complete nucleotide sequence of SV40 DNA
Walter Fiers,Roland Contreras,Guy Haegeman,R. Rogiers,A. Van de Voorde,H. Van Heuverswyn,J. Van Herreweghe,Guido Volckaert,M. Ysebaert +8 more
TL;DR: The determination of the total 5,224 base-pair DNA sequence of the virus SV40 has enabled us to locate precisely the known genes on the genome.
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The genome of simian virus 40
V.B. Reddy,B Thimmappaya,Ravi Dhar,K N Subramanian,B. S. Zain,Julian Pan,Prabhat K. Ghosh,M. L. Celma,Sherman M. Weissman +8 more
TL;DR: The nucleotide sequence of SV40 DNA was determined, and the sequence was correlated with known genes of the virus and with the structure of viral messenger RNA's.