Journal ArticleDOI
Microfluidic Hydrodynamic Cell Separation: A Review
Zhigang Wu,Klas Hjort +1 more
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TLDR
In this article, the basic ideas and fluid mechanics for the hydrodynamic interaction of a particle in a microfluidic system are presented. And different kinds of devices are introduced with detailed descriptions of their mechanisms, designs and performances.Abstract:
Microfluidic continuous cell separation based on hydrodynamic interaction in a microfluidic channel has attracted attention because of its robustness, high throughput and cell viability This paper systematically gives an overview on recent advances in hydrodynamic particle and cell separation in microfluidic devices It presents the basic ideas and fluid mechanics for the hydrodynamic interaction of a particle in a microfluidic system Secondly, different kinds of devices are introduced with detailed descriptions of their mechanisms, designs and performances Finally, the review addresses some practical issues of microfluidic sorting devices for use in biological or medical studiesread more
Citations
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Passive blood plasma separation at the microscale: a review of design principles and microdevices
TL;DR: In this article, the authors present an extensive review of relevant biophysical laws, along with experimental details of various passive separation techniques and devices exploiting these physical effects, and compare the relative performances, and the advantages and disadvantages of microdevices discussed in the literature.
Journal ArticleDOI
Circulating tumor-cell detection and capture using microfluidic devices
TL;DR: This article critically review the most promising hydrodynamic, dielectrophoretic and magnetic force-based microfluidic CTC-capture devices utilizing the physical and biochemical properties of CTCs.
Journal ArticleDOI
Microfluidic bio-particle manipulation for biotechnology
TL;DR: In this paper, a review of different techniques and the recent applications regarding the micro-fluidic bio-particle manipulation for different biotechnology applications are presented, and challenges and the future research directions for micro-particles manipulation are addressed.
Journal ArticleDOI
Detection of Circulating Tumor Cells Using Microfluidics
Tiberiu Burinaru,Marioara Avram,Andrei Avram,Cătălin Mărculescu,Bianca Ţîncu,Vasilica Ţucureanu,Alina Matei,Manuella Militaru +7 more
TL;DR: A review of devices that detect and capture CTCs using different cell properties (surface markers, size, deformability, electrical properties, etc.) and discusses the process of tumor cell dissemination, the biology of C TCs, epithelial-mesenchymal transition (EMT), and several challenges and clinical applications of CTC detection.
Journal ArticleDOI
Vertical Hydrodynamic Focusing and Continuous Acoustofluidic Separation of Particles via Upward Migration.
TL;DR: The proposed particle separation device offers high‐throughput operation with purity >97% and recovery rate >99%.
References
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Journal ArticleDOI
Microfluidic devices for size-dependent separation of liver cells
TL;DR: The results showed that the presented microfluidic devices are low-cost and suitable for clinical use, and capable of highly functional separation with relatively high-speed processing.
Journal ArticleDOI
Recent advances in microparticle continuous separation
TL;DR: Recent techniques of micron-sized particle separation within microsystems are described with emphasis on five different categories: optical, magnetic, fluidic-only, electrical and minor separation methods.
Journal ArticleDOI
Filter-based microfluidic device as a platform for immunofluorescent assay of microbial cells.
TL;DR: A filter-based microfluidic device was combined with immunofluorescent labeling as a platform to rapidly detect microbial cells and those target cells with a fluorescent signal-to-noise ratio of 12 could be microscopically observed at single-cell level within 2 to 5 min prior to secondary washing.
Journal ArticleDOI
Using ratchets and sorters to fractionate motile cells of Escherichia coli by length
S. Elizabeth Hulme,Willow R. DiLuzio,Sergey S. Shevkoplyas,Linda Turner,Michael Mayer,Howard C. Berg,George M. Whitesides +6 more
TL;DR: A composite agar/PDMS device for enriching short cells in a population of motile Escherichia coli was fabricated, which incorporated ratcheting microchannels, which directed the motion of swimming cells of E. coli through the device, and three sorting junctions, which isolated successively shorter populations of bacteria.
Journal ArticleDOI
Microfluidic isolation of leukocytes from whole blood for phenotype and gene expression analysis.
Palaniappan Sethu,Lyle L. Moldawer,Michael N. Mindrinos,Philip O. Scumpia,Cynthia L. Tannahill,Julie Wilhelmy,Philip A. Efron,Bernard H. Brownstein,Ronald G. Tompkins,Mehmet Toner +9 more
TL;DR: The results demonstrate that this system can isolate unactivated leukocytes from small blood samples without any significant loss, which permits more information to be obtained from subsequent analysis, and will be readily applicable to clinical settings.