Molecular and functional characterization of a novel low-affinity cation transporter (LCT1) in higher plants
TLDR
The structure of this higher plant ion transport protein is unique and contains PEST sequences, which suggests that the protein contains 8-10 transmembrane helices and a hydrophilic amino terminus containing sequences enriched in Pro, Ser, Thr, and Glu (PEST).Abstract:
The transport of cations across membranes in higher plants plays an essential role in many physiological processes including mineral nutrition, cell expansion, and the transduction of environmental signals In higher plants the coordinated expression of transport mechanisms is essential for specialized cellular processes and for adaptation to variable environmental conditions To understand the molecular basis of cation transport in plant roots, a Triticum aestivum cDNA library was used to complement a yeast mutant deficient in potassium (K+) uptake Two genes were cloned that complemented the mutant: HKT1 and a novel cDNA described in this report encoding a cation transporter, LCT1 (low-affinity cation transporter) Analysis of the secondary structure of LCT1 suggests that the protein contains 8–10 transmembrane helices and a hydrophilic amino terminus containing sequences enriched in Pro, Ser, Thr, and Glu (PEST) The transporter activity was assayed using radioactive isotopes in yeast cells expressing the cDNA LCT1 mediated low-affinity uptake of the cations Rb+ and Na+, and possibly allowed Ca2+ but not Zn2+ uptake LCT1 is expressed in low abundance in wheat roots and leaves The precise functional role of this cation transporter is not known, although the competitive inhibition of cation uptake by Ca2+ has parallels to whole plant and molecular studies that have shown the important role of Ca2+ in reducing Na+ uptake and ameliorating Na+ toxicity The structure of this higher plant ion transport protein is unique and contains PEST sequencesread more
Citations
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Plant salt tolerance
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Na+ Tolerance and Na+ Transport in Higher Plants
Mark Tester,Romola Davenport +1 more
TL;DR: This work suggests that equally important in a wide range of conditions are processes involving the management of Na(+) movements within the plant, and requires more knowledge of cell-specific transport processes and the consequences of manipulation of transporters and signalling elements in specific cell types.
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The Arabidopsis thaliana salt tolerance gene SOS1 encodes a putative Na+/H+ antiporter.
TL;DR: In this article, the SOS1 (Salt Overly Sensitive 1) locus is cloned and predicted to encode a 127-kDa protein with 12 transmembrane domains in the N-terminal part and a long hydrophilic cytoplasmic tail.
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Molecular mechanisms of plant metal tolerance and homeostasis.
TL;DR: Recent progress in the molecular understanding of plant metal homeostasis and tolerance is reviewed and a number of uptake transporters have been cloned as well as candidates for the vacuolar sequestration of metals are identified.
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A long way ahead: understanding and engineering plant metal accumulation.
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References
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A simple method for displaying the hydropathic character of a protein
Jack Kyte,Russell F. Doolittle +1 more
TL;DR: A computer program that progressively evaluates the hydrophilicity and hydrophobicity of a protein along its amino acid sequence has been devised and its simplicity and its graphic nature make it a very useful tool for the evaluation of protein structures.
Book
The Mineral Nutrition of Higher Plants
M. H. Martin,H. Marschner +1 more
TL;DR: This chapter discusses the relationship between Mineral Nutrition and Plant Diseases and Pests, and the Soil-Root Interface (Rhizosphere) in Relation to Mineral Nutrition.
Book
Mineral Nutrition of Higher Plants
TL;DR: In this article, the authors discuss the relationship between mineral nutrition and plant diseases and pests, and diagnose deficiency and toxicity of mineral nutrients in leaves and other aerial parts of a plant.
Journal ArticleDOI
Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis
TL;DR: The rapid degradation of injected alpha- and beta-casein as well as the inverse correlation of PEST regions with intracellular stability indicate that the presence of these regions can result in the rapid intrace cellular degradation of the proteins containing them.