Journal ArticleDOI
Molecular approaches to the identification of Bulinus species in south-west Nigeria and observations on natural snail infections with schistosomes.
O. P. Akinwale,Richard A. Kane,David Rollinson,J. R. Stothard,M. B. Ajayi,D.O. Akande,M.O. Ogungbemi,C. Duker,P. V. Gyang,Monsuru A. Adeleke +9 more
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TLDR
The results showed that the majority of Bulinus samples tested belonged to the species Bulinus truncatus while only two were Bulinus globosus, and Sequencing of the partial schistosome its from a small subset of snail samples suggested that some snails were either penetrated by both Schistosoma haematobium and SchistOSoma bovis miracidia or hybrid miracidia formed from the two species.Abstract:
The current study considers the distribution of a small sample of 138 Bulinus snails, across 28 localities within eight Nigerian states. Snails were identified using a combination of molecular methods involving both DNA sequencing of a partial cytochrome oxidase subunit 1 (cox1) fragment and restriction profiles obtained from ribosomal internal transcribed spacer (its) amplicons. The results showed that the majority of Bulinus samples tested belonged to the species Bulinus truncatus while only two were Bulinus globosus. The use of RsaI restriction endonuclease to cleave the ribosomal its of Bulinus, as a method of species identification, was adopted for the majority of samples, this being a quicker and cheaper method better suited to small laboratory environments. Polymerase chain reaction (PCR) amplification of the schistosome Dra1 repeat within each of the collected Bulinus samples was employed to determine the extent and distribution of infected snails within the sample areas. Successful amplification of the Dra1 repeat demonstrated that 29.7% of snails were infected with schistosomes. Sequencing of the partial schistosome its from a small subset of snail samples suggested that some snails were either penetrated by both Schistosoma haematobium and Schistosoma bovis miracidia or hybrid miracidia formed from the two species.read more
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Time to set the agenda for schistosomiasis elimination.
David Rollinson,Stefanie Knopp,Stefanie Knopp,Sarah Levitz,J. Russell Stothard,Louis-Albert Tchuem Tchuenté,Amadou Garba,Amadou Garba,Khalfan A. Mohammed,Nadine Schur,Nadine Schur,Bobbie Person,Daniel G. Colley,Jürg Utzinger,Jürg Utzinger +14 more
TL;DR: An agenda for the elimination of schistosomiasis would aim to identify the gaps in knowledge, and define the tools, strategies and guidelines that will help national control programmes move towards elimination, including an internationally accepted mechanism that allows verification/confirmation of elimination.
Journal ArticleDOI
Advances in the Diagnosis of Human Schistosomiasis
TL;DR: Careful and rigorous validation of these assays and their cost-effectiveness will be needed, however, prior to their adoption in support of policy decisions for national public health programs aimed at the control and elimination of schistosomiasis.
Journal ArticleDOI
Diagnostics for schistosomiasis in Africa and Arabia: a review of present options in control and future needs for elimination
J. Russell Stothard,Michelle C. Stanton,Amaya L. Bustinduy,José Carlos Sousa-Figueiredo,Govert J. van Dam,Martha Betson,David Waterhouse,Steve A. Ward,Fiona Allan,Amir Hassan,Mohammad A. Al-Helal,Ziad A. Memish,David Rollinson +12 more
TL;DR: Current options for diagnosis of schistosomiasis at different levels are reviewed, highlighting several strengths and weaknesses therein and it is proposed that more cost-effective diagnostics and clinical staging algorithms are needed.
Journal ArticleDOI
Prevalence and distribution of schistosomiasis in human, livestock, and snail populations in northern Senegal: a One Health epidemiological study of a multi-host system.
Elsa Léger,Elsa Léger,Anna Borlase,Cheikh Fall,Nicolas D. Diouf,Samba D Diop,Lucy Yasenev,Stefano Catalano,Stefano Catalano,Cheikh Thiam,Alassane N’Diaye,Aidan M. Emery,Alice Morrell,Muriel Rabone,Muriel Rabone,Momar Ndao,Babacar Faye,David Rollinson,David Rollinson,James W. Rudge,Mariama Sène,Joanne P. Webster,Joanne P. Webster +22 more
TL;DR: The prevalence, distribution, and multi-host, multiparasite transmission cycle of Haematobium group schistosomiasis in Senegal, West Africa is investigated to investigate the potential zoonotic risk posed by livestock Schistosoma species via viable hybridisation in sub-Saharan Africa.
Journal ArticleDOI
Prevalence and risk factors of schistosomiasis among hausa communities in kano state, nigeria
Salwa Dawaki,Hesham M. Al-Mekhlafi,Hesham M. Al-Mekhlafi,Init Ithoi,Jamaiah Ibrahim,Awatif Mohammed Abdulsalam,Abdulhamid Ahmed,Hany Sady,Wahib M. Atroosh,Mona A. Al-Areeqi,Fatin Nur Elyana,Nabil A. Nasr,Johari Surin +12 more
TL;DR: It is revealed that schistosomiasis is still prevalent among Hausa communities in Nigeria and mass drug administration, health education and community mobilization are imperative strategies to significantly reduce the prevalence and morbidity of schistOSomiasis in these communities.
References
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