Journal ArticleDOI
Molecular farming in plants: host systems and expression technology.
TLDR
Plants provide an inexpensive and convenient system for the large-scale production of valuable recombinant proteins and will only realize its huge potential if constraints are removed through rigorous and detailed science-based studies.About:
This article is published in Trends in Biotechnology.The article was published on 2003-12-01. It has received 650 citations till now.read more
Citations
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Journal ArticleDOI
Plant-based production of biopharmaceuticals.
TL;DR: The first plant-derived recombinant pharmaceutical proteins are reaching the final stages of clinical evaluation, and many more are in the development pipeline, as shown by the continuing commercial development of novel plant-based expression platforms.
Journal ArticleDOI
Plant cell cultures for the production of recombinant proteins
TL;DR: Although no recombinant proteins have yet been produced commercially using plant cell cultures, there have been many proof-of-principle studies and several companies are investigating the commercial feasibility of such production systems.
Journal ArticleDOI
In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning
TL;DR: The data demonstrate that Cd perturbs the DNA methylation status through the involvement of a specific methyltransferase, linked to nuclear chromatin reconfiguration likely to establish a new balance of expressed/repressed chromatin.
Journal ArticleDOI
Plant protoplasts: status and biotechnological perspectives
TL;DR: Novel approaches to maximise the efficiency of protoplast-to-plant systems include techniques already well established for animal and microbial cells, such as electrostimulation and exposure of protoplasts to surfactants and respiratory gas carriers, especially perfluorochemicals and hemoglobin.
Journal ArticleDOI
Sowing the seeds of success: pharmaceutical proteins from plants.
TL;DR: The use of seeds for the production of pharmaceutical proteins, particularly replacement human proteins, recombinant antibodies and (oral) vaccines, has been explored, and the first commercial products have already reached the market.
References
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Journal ArticleDOI
Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants.
TL;DR: A set of plasmids has been constructed utilizing the promoter, 5′ untranslated exon, and first intron of the maize ubiquitin (Ubi-1) gene to drive expression of protein coding sequences of choice to provide expression of biotechnologically important protein products in transgenic plants.
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An Agrobacterium-mediated transient gene expression system for intact leaves
TL;DR: An efficient and reproducible Agrobacterium -mediated transient gene expression system for intact leaf tissue was developed and similar results were obtained with other plant species, such as Phaseolus acutifolius, poplar, and tobacco.
Journal ArticleDOI
Generation and assembly of secretory antibodies in plants.
Julian K.-C. Ma,A. Hiatt,Mich B. Hein,Nicholas D. Vine,Fei Wang,P. Stabila,C. van Dolleweerd,Keith E. Mostov,Thomas Lehner +8 more
TL;DR: Transgenic plants may be suitable for large-scale production of recombinant secretory immunoglobulin A for passive mucosal immunotherapy because plant cells also possess the requisite mechanisms for assembly and expression of other complex recombinant protein molecules.
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N-glycoprotein biosynthesis in plants : recent developments and future trends
Patrice Lerouge,Marion Cabanes-Macheteau,Catherine Rayon,Anne-Catherine Fischette-Lainé,Véronique Gomord,Loïc Faye +5 more
TL;DR: The potential and limits of plant cells as a factory for the production of mammalian glycoproteins will be illustrated and fast progress is currently made by using N-linked glycan structures as milestones of the intracellular transport of proteins along the plant secretory pathway.
Journal ArticleDOI
Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato.
Carol O. Tacket,Hugh S. Mason,Genevieve Losonsky,John D. Clements,Myron M. Levine,Charles J. Arntzen +5 more
TL;DR: The present study was conducted as a proof of principle to determine if humans would also develop a serum and/or mucosal immune response to an antigen delivered in an uncooked foodstuff.