Journal ArticleDOI
Molecular Monolayers and Interfacial Electron Transfer of Pseudomonas aeruginosa Azurin on Au(111)
Qijin Chi,Jingdong Zhang,Jens Ulrik Nielsen,Esben Peter Friis,Ib Chorkendorff,Gerard W. Canters,Jens Enevold Thaulov Andersen,Jens Ulstrup +7 more
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TLDR
In this paper, the formation and characterization of molecular monolayers of the blue copper protein Pseudomonas aeruginosa azurin on Au(111) in aqueous ammonium acetate solution was provided.Abstract:
We provide a comprehensive approach to the formation and characterization of molecular monolayers of the blue copper protein Pseudomonas aeruginosa azurin on Au(111) in aqueous ammonium acetate solution. Main issues are adsorption patterns, reductive desorption, properties of the double layer, and long-range electrochemical electron transfer between the electrode and the copper center. Voltammetry, electrochemical impedance spectroscopy (EIS), in situ scanning tunneling microscopy (STM), and X-ray photoelectron spectroscopy (XPS) have been employed to disclose features of these issues. Zn-substituted azurin, cystine, and 1-butanethiol are investigated for comparison. Cyclic voltammetric and capacitance measurements show qualitatively that azurin is adsorbed at submicromolar concentrations over a broad potential range. The characteristics of reductive desorption suggest that azurin is adsorbed via its disulfide group to form a monolayer. The adsorption of this protein on Au(111) via a gold−sulfur binding m...read more
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Molybdenum sulfides—efficient and viable materials for electro - and photoelectrocatalytic hydrogen evolution
TL;DR: In this paper, the authors present the electrocatalytic properties of a highly conductive MoS2 hybrid material, and demonstrate that the origin of the HER activity is closely related to the amount of edges in the layered MOS2.
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Direct Electrochemistry of Redox Enzymes as a Tool for Mechanistic Studies
TL;DR: This review regards the use of dynamic electrochemistry to study the mechanism of redox enzymes, with exclusive emphasis on the configuration where the protein is adsorbed onto an electrode and electron tranfer is direct.
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Microbial Biofilm Voltammetry: Direct Electrochemical Characterization of Catalytic Electrode-Attached Biofilms
TL;DR: These techniques provide the basis for cataloging quantifiable, defined electron transfer phenotypes as a function of potential, electrode material, growth phase, and culture conditions and provide a framework for comparisons with other species or communities.
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Electrochemistry of Nonconjugated Proteins and Glycoproteins. Toward Sensors for Biomedicine and Glycomics
TL;DR: In this review, it is shown that in the recent years a significant progress was done in the EC analysis of practically all proteins, based on electroactivity of amino acid (aa) residues in proteins.
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DFT Study of Cysteine Adsorption on Au(111)
TL;DR: In this article, the adsorption of the cysteine amino acid (H−SCβH2−CαH−NH 2−COOH) on the (111) surface of gold is studied by means of periodic density functional calculations.
References
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MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures
TL;DR: The MOLSCRIPT program as discussed by the authors produces plots of protein structures using several different kinds of representations, including simple wire models, ball-and-stick models, CPK models and text labels.
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Supported Membranes: Scientific and Practical Applications
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The electrochemical desorption of n-alkanethiol monolayers from polycrystalline Au and Ag electrodes
TL;DR: In this article, the surface coverage of the oxidized n-alkanethiol species is shown to be 9.3×10−10 mol/cm2 and 7.0 × 10−10 cm2 on Au and Ag, respectively.
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Molecular Spectroscopy by Means of ESCA II. Sulfur compounds. Correlation of electron binding energy with structure
TL;DR: In this article, a correlation has been established between the observed position of inner electron lines of sulfur and structure, and the influence of structure on the electron binding energies is discussed in terms of a calculated atomic charge, based on the concepts of electronegativity and partial ionic character of bonds.