Journal ArticleDOI
Molecular types and antibiotic resistance of Staphylococcus aureus isolates from bovine mastitis in a single herd in China.
TLDR
The molecular diversity, antibiotic resistance patterns and presence of resistance genes were determined in Staphylococcus aureus isolates from cases of bovine mastitis in a dairy cattle herd in China.Abstract:
The molecular diversity, antibiotic resistance patterns and presence of resistance genes were determined in Staphylococcus aureus isolates from cases of bovine mastitis in a dairy cattle herd in China. Multiple locus variable number tandem repeat analysis was used for molecular typing. Resistance was determined through minimum inhibitory concentrations and resistance genes were detected by PCR. There was low molecular diversity; one predominant strain (type I) accounted for the majority of cases of S. aureus mastitis in the herd and this strain had a high frequency of resistance to penicillin and tetracycline. The most prevalent resistance genes were blaZ, ermC and tetM.read more
Citations
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Prevalence and antimicrobial resistance of Staphylococcus aureus isolated from raw milk and dairy products
TL;DR: Consumption of raw milk and dairy products are revealed as a potential risk of foodborne infection in this region and a high frequency of blaZ and tetM resistance genes was found in S. aureus isolates.
Journal ArticleDOI
Bovine mastitis Staphylococcus aureus: antibiotic susceptibility profile, resistance genes and molecular typing of methicillin-resistant and methicillin-sensitive strains in China.
TL;DR: The results revealed that the resistance of S. aureus is becoming more complicated by changes in multi-drug resistance mechanism and appearance of mecA-negative MRSA isolates, and importantly, MRSA-IV strains in different MLST types are emerging.
Journal ArticleDOI
Coagulase-negative staphylococci (CoNS) isolated from ready-to-eat food of animal origin--phenotypic and genotypic antibiotic resistance
Wioleta Chajęcka-Wierzchowska,Anna Zadernowska,Beata Nalepa,Magda Sierpińska,Łucja Łaniewska-Trokenheim +4 more
TL;DR: Although coagulase-negative staphylococci are not classical food poisoning bacteria, its presence in food could be of public health significance due to the possible spread of antibiotic resistance.
Journal ArticleDOI
Antimicrobial resistance profiles of 5 common bovine mastitis pathogens in large Chinese dairy herds.
Jia Cheng,Weijie Qu,Herman W. Barkema,Diego B. Nobrega,Jian Gao,Gang Liu,Jeroen De Buck,John P. Kastelic,Hong Sun,Bo Han +9 more
TL;DR: Prevalence of AMR in mastitis pathogens was high on large Chinese dairy farms, potentially jeopardizing both antimicrobial efficacy and public health, and highlighted the need for improvements in antimicrobial stewardship and infection control programs in largeChinese dairy farms to reduce emergence of AMr.
Journal ArticleDOI
Enterotoxigenicity and Antimicrobial Resistance of Staphylococcus aureus Isolated from Retail Food in China.
Wei Wang,Zulqarnain Baloch,Tao Jiang,Cunshan Zhang,Zixin Peng,Fengqin Li,Séamus Fanning,Séamus Fanning,Séamus Fanning,Aiguo Ma,Jin Xu +10 more
TL;DR: It is important to monitor the antimicrobial susceptibility and enterotoxigenicity of MDR S. aureus and MRSA in the food chain and to use these data to develop food safety measures, designed to reduce the contamination and transmission of this bacterium.
References
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Journal ArticleDOI
Molecular Detection of Antimicrobial Resistance
TL;DR: Although molecular techniques for the detection of antimicrobial resistance clearly are winning a place in routine diagnostics, phenotypic assays are still the method of choice for most resistance determinations.
Journal ArticleDOI
New method for typing Staphylococcus aureus strains: multiple-locus variable-number tandem repeat analysis of polymorphism and genetic relationships of clinical isolates.
Artur Sabat,Jolanta Krzysztoń-Russjan,Wojciech Strzalka,Renata Filipek,Klaudia Kosowska,Waleria Hryniewicz,James Travis,Jan Potempa,Jan Potempa +8 more
TL;DR: The PCR-based methodology applied to multiple-locus variable numbers of tandem repeat (VNTR) analysis was recently shown to be a useful technique for the molecular typing of clinical isolates of several bacterial species and it was shown that the method allows typing of S. aureus strains with the discriminatory power and reproducibility of pulsed-field gel electrophoresis.
Journal ArticleDOI
Multiplex PCR assays for the detection of clinically relevant antibiotic resistance genes in staphylococci isolated from patients infected after cardiac surgery
Francis Martineau,François J. Picard,Louis Grenier,Paul H. Roy,Marc Ouellette,Michel G. Bergeron +5 more
TL;DR: This study suggests that the incidence and distribution of the tested clinically relevant antibiotic resistance genes in staphylococci associated with infections after cardiac surgery do not differ from those in strains from other infections.
Journal ArticleDOI
Regulation of antibiotic resistance in Staphylococcus aureus.
TL;DR: The driving force behind resistance formation is antibiotic stress, and under clinical conditions selection for resistance is continuously competing with selection for bacterial fitness.
Journal ArticleDOI
Molecular Typing and Distribution of Staphylococcus aureus Isolates in Eastern Canadian Dairy Herds
Parviz M. Sabour,Jason J. Gill,D. Lepp,Jennifer C. Pacan,Rafiq Ahmed,R. Dingwell,Ken E. Leslie +6 more
TL;DR: Macrorestriction analysis of SmaI-digested chromosomal DNA, using pulsed field gel electrophoresis (PFGE) was performed to type and estimate genetic relationships among 288 Staphylococcus aureus isolates recovered from 58 Eastern Canadian dairy herds as mentioned in this paper.