Monitoring of Orientation in Molecular Ensembles by Polarization Sensitive Nonlinear Microscopy
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TLDR
In this paper, a high-resolution two-photon excitation microscopy study was conducted to investigate the orientational distributions of molecular ensembles at room temperature. Butt et al. presented a more general approach that combines twophoton fluorescence (TPF) and second harmonic generation (SHG).Abstract:
We present high resolution two-photon excitation microscopy studies combining two-photon fluorescence (TPF) and second harmonic generation (SHG) in order to probe orientational distributions of molecular ensembles at room temperature. A detailed polarization analysis of TPF and SHG signals is used in order to unravel the parameters of the molecular orientational statistical distribution, using a technique which can be extended and generalized to a broad variety of molecular arrangements. A polymer film containing molecules active for TPF and/or SHG emission is studied as a model system. Polarized TPF is shown to provide information on specific properties pertaining to incoherent emission in molecular media, such as excitation transfer. SHG, being highly sensitive to a slight departure from centrosymmetry such as induced by an external electric field in the medium, complements TPF. The response of each signal to a variable excitation polarization allows investigation of molecular behavior in complex enviro...read more
Citations
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Polarization-resolved nonlinear microscopy: application to structural molecular and biological imaging
TL;DR: In this article, the polarization-dependent properties of different optical contrasts widely used today in imaging, applied to biology and biomedical diagnostics, are analyzed and compared. And the essential properties of the polarization dependence of optical processes such as two-photon fluorescence, nonlinear coherent effects in the non-resonant as well as vibrational-resonsant regimes, and analyze how they can be exploited to provide information on the molecular orientational organization in a biological sample.
Journal ArticleDOI
Photostable second-harmonic generation from a single KTiOPO4 nanocrystal for nonlinear microscopy.
Loc Le Xuan,Chunyuan Zhou,Abdallah Slablab,Dominique Chauvat,Cédric Tard,Sandrine Perruchas,Thierry Gacoin,Philippe Villeval,Jean-François Roch +8 more
TL;DR: A complementary approach consists of considering a well-known SHG-active bulk material and investigating its properties in nanoparticle form, including potassium titanyl phosphate, a widely used nonlinear crystal.
Journal ArticleDOI
Second-harmonic generation from a single core/shell quantum dot.
TL;DR: Coherent SHG from single core/shell CdTe/CdS nanocrystals with a diameter of 10 to 15 nm is reported, and the nanocrystal excitation spectrum reveals resonances in the nonlinear efficiency with an overall maximum at about 970 nm.
Journal ArticleDOI
In vivo, pixel-resolution mapping of thick filaments' orientation in nonfibrilar muscle using polarization-sensitive second harmonic generation microscopy.
Sotiris Psilodimitrakopoulos,Susana I. C. O. Santos,Ivan Amat-Roldan,Anisha Thayil,David Artigas,Pablo Loza-Alvarez +5 more
TL;DR: This methodology is presented as a filtering mechanism to uncover biological information unreachable by common intensity SHG microscopy and a method to experimentally retrieve the distribution of the effective orientation of active SHG molecules is proposed and tested.
Journal ArticleDOI
Limits on fluorescence detected circular dichroism of single helicene molecules.
TL;DR: The dissymmetry factors of single chiral fluorophores are found not to differ significantly from the bulk value of |g| < 10(-4) at 457 nm, and compensation should be made to avoid artifacts brought by linear dichroism in single immobilized molecules.
References
More filters
Journal ArticleDOI
Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
Journal ArticleDOI
Association of human papillomavirus types 16 and 18 E6 proteins with p53.
TL;DR: This study shows that the E6 protein of HPV-16 is capable of binding to the cellular p53 protein, providing further evidence that the human papillomaviruses, the adenovirus type 5, and SV40 may effect similar cellular pathways in transformation.
Journal ArticleDOI
Design of Organic Molecules with Large Two-Photon Absorption Cross Sections
Marius A. Albota,David Beljonne,Jean-Luc Brédas,J. E. Ehrlich,Jia Ying Fu,Ahmed A. Heikal,Samuel E. Hess,Thierry Kogej,Michael D. Levin,Seth R. Marder,D. McCord-Maughon,Joseph W. Perry,H. Rockel,Mariacristina Rumi,Girija Subramaniam,Watt W. Webb,Xiang Li Wu,Chris Xu +17 more
TL;DR: The combination of large delta and high fluorescence quantum yield or triplet yield exhibited by molecules developed here offers potential for unprecedented brightness in two-photon fluorescent imaging or enhanced photosensitivity in two -photon sensitization, respectively.
Journal ArticleDOI
Near-Field Second-Harmonic Generation Induced by Local Field Enhancement
TL;DR: A theoretical model for the excitation and emission of SH radiation at the tip is developed and it is found that this source can be represented by a single on-axis oscillating dipole.
Journal ArticleDOI
Nonlinear scanning laser microscopy by third harmonic generation
TL;DR: In this article, the authors used the third harmonic generation near the focal point of a tightly focused beam to probe microscopical structures of transparent samples, which can resolve interfaces and inhomogeneities with axial resolution comparable to the confocal length of the beam.