Journal ArticleDOI
Multiple forms of β-galactosidase from the germinating seeds of Vigna radiata
TLDR
Four forms of β-galactosidase were isolated and partially purified from the cotyledons of germinating seeds of Vigna radiata by ammonium sulphate fractionation and ion exchange chromatography through DEAE-cellulose and CM- cellulose columns, showing similar pH optima and temperature optima but differed from each other in ionic charge, kinetic parameters, activation energy and sensitivity towards heat.About:
This article is published in Phytochemistry.The article was published on 1990-01-01. It has received 11 citations till now. The article focuses on the topics: Galactoside.read more
Citations
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Journal ArticleDOI
β-Galactosidase and Its Significance in Ripening of “Saijyo” Japanese Persimmon Fruit
TL;DR: The fruit extracts of ripening cv.
Journal ArticleDOI
An extracellular β-galactosidase secreted from cell suspension cultures of carrot. Its purification and involvement in cell wall-polysaccharide hydrolysis
Haruyoshi Konno,Kenji Katoh +1 more
TL;DR: The enzyme acted on citrus galactan and larchwood arabinogalactan in an exo-fashion, and was slightly involved in the hydrolysis of an acidic pectic polymer containing arabinosyl and galactosyl residues and in the breakdown of cell walls isolated from carrot cell cultures.
Journal ArticleDOI
Purification and Characterization of Extracellular β-Galactosidase Secreted by Supension Cultured Rice (Oryza sativa L.) Cells
TL;DR: A β-galactosidase was purified 1300-fold by lactosyl-Sepharose 4B and Sephacryl S-200 column chromatographies from the cultured medium of a rice-cell suspension and released galactose from galactoxyloglucan and pectic galactans.
Journal ArticleDOI
Multiple forms of cotyledonary b-galactosidases from Vigna unguiculata quiescent seeds
TL;DR: Isoelectric focusing in polyacrylamide slab gel of these enzyme-proteins suggest that cotyledons of Pitiuba cowpea quiescent seeds possess four isoforms of b-galactosidases.
Journal ArticleDOI
Isolation and partial purification of beta-galactosidases from cotyledons of two cowpea cultivars
Joaquim Enéas-Filho,Gislainy Karla da Costa Barbosa,Fabrício Bonfim Sudério,José Tarquinio Prisco,Enéas Gomes-Filho +4 more
TL;DR: The results have shown that the observed differences in b-galactosidases from the cotyledons of quiescent seeds were not sufficient to relate them to stress tolerance.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Estimation of the molecular weights of proteins by Sephadex gel-filtration.
TL;DR: The results are similar to those of previous studies, where the objective was to establish a cause-and-effect relationship, rather than a straightforward relationship between the number of cells and the content of the molecule.