Journal ArticleDOI
Plant β-galactosidases: Purification by affinity chromatography and properties
TLDR
β-Galactosidases from seven plants have been extensively purified by affinity chromatography of an ammonium sulphate concentrate of the respective extracts through a column of Lactosyl-Sepharose, showing charge heterogeneity containing from two to 11 enzymically active isoelectric forms.About:
This article is published in Phytochemistry.The article was published on 1989-01-01. It has received 36 citations till now. The article focuses on the topics: Affinity chromatography & Isoelectric point.read more
Citations
More filters
Journal ArticleDOI
Kiwifruit β-galactosidase: Isolation and activity against specific fruit cell-wall polysaccharides
TL;DR: Results indicate that the β-galactosidase of this study is solely responsible for the removal of galactose from the cell wall during ripening, and its in-vivo activity must be much greater than that observed in- vitro.
Journal ArticleDOI
Apple [beta]-Galactosidase (Activity against Cell Wall Polysaccharides and Characterization of a Related cDNA Clone)
TL;DR: A [beta]-galactosidase was purified from cortical tissue of ripe apples (Malus domestica Borkh. cv Granny Smith) using a procedure involving affinity chromatography on lactosyl-Sepharose as discussed by the authors.
Journal ArticleDOI
Down‐regulation of a ripening‐related β‐galactosidase gene (TBG1) in transgenic tomato fruits
Annette Teresa Carey,David L. Smith,Elizabeth Harrison,Colin Roger Bird,Kenneth C. Gross,Graham B. Seymour,Gregory A. Tucker +6 more
TL;DR: It was concluded that during the ripening of tomato fruits a family of beta-galactosidases capable of degrading cell wall galactans are active and down-regulation of TBG1 message to 10% was insufficient to alter the degree of galactan degradation.
Journal ArticleDOI
Purification and characterization of isoforms of β-galactosidases in mung bean seedlings
TL;DR: Double immunodiffusion analysis indicated thatbeta-galactosidases I, II, III and V are immunologically similar to each other, while beta-galactsosidase IV shares partially identical antigenic determinants with the other four isoforms.
References
More filters
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity
TL;DR: It was found that treatment of gels with dithiothreitol prior to impregnation with silver nitrate results in more reproducible staining patterns that are also qualitatively similar to those obtained with Coomassie blue.