Journal ArticleDOI
Multiplex PCR for detection of acquired carbapenemase genes
TLDR
A rapid and reliable PCR-based technique was developed for detection of genes encoding carbapenemases belonging to different classes using optimized conditions, with PCR giving distinct amplicon sizes corresponding to the different genes for each mixture.About:
This article is published in Diagnostic Microbiology and Infectious Disease.The article was published on 2011-05-01. It has received 1438 citations till now.read more
Citations
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Journal ArticleDOI
Global spread of Carbapenemase-producing Enterobacteriaceae.
TL;DR: These resistance traits have been identified among nosocomial and community-acquired infections and are being investigated for use in drug discovery and development.
Journal ArticleDOI
Carbapenemases in Klebsiella pneumoniae and Other Enterobacteriaceae: an Evolving Crisis of Global Dimensions
Leonidas S. Tzouvelekis,Antonios Markogiannakis,Mina Psichogiou,Panayotis T. Tassios,George L. Daikos +4 more
TL;DR: Therapeutic options for treating carbapenemase-producing enterobacteria (CPE) infect mainly hospitalized patients but also have been spreading in long-term care facilities, and pharmacodynamic simulations and experimental infections indicate that modification of the current patterns of carbapENem use against CPE warrants further attention.
Journal ArticleDOI
OXA-48-like carbapenemases: the phantom menace
TL;DR: Since many OXA-48-like producers do not exhibit resistance to broad-spectrum cephalosporins, or only decreased susceptibility to carbapenems, their recognition and detection can be challenging.
Journal ArticleDOI
Identification and screening of carbapenemase-producing Enterobacteriaceae
Patrice Nordmann,Marek Gniadkowski,Christian G. Giske,Laurent Poirel,Neil Woodford,Vivi Miriagou +5 more
TL;DR: Phenotypic and molecular-based techniques are able to identify these carbapenemase producers, although with variable efficiencies, and the detection of carriers still relies mostly on the use of screening culture media.
Journal ArticleDOI
Genetic Features of blaNDM-1-Positive Enterobacteriaceae
TL;DR: The blaNDM-1 gene was carried by different plasmid types (IncA/C, IncF, IncL/M, or untypeable) and was likely chromosome borne in two isolates and coharbored a variety of resistance determinants, including β-lactamase genes, quinolone resistance genes, and 16S RNA methylase genes.
References
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Journal ArticleDOI
Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study
Karthikeyan Kumarasamy,Mark Toleman,Timothy R. Walsh,Jay Bagaria,Fafhana Butt,Ravikumar Balakrishnan,Uma Chaudhary,Michel Doumith,Christian G. Giske,Seema Irfan,Padma Krishnan,Anil Kumar,Sunil Maharjan,Shazad Mushtaq,Tabassum Noorie,David L. Paterson,A. Pearson,Claire Perry,Rachel Pike,Bhargavi Rao,Ujjwayini Ray,Jayanta B. Sarma,Madhu Sharma,Elizabeth Sheridan,M A Thirunarayan,Jane F. Turton,Supriya Upadhyay,Marina Warner,William Welfare,David M. Livermore,Neil Woodford +30 more
TL;DR: The prevalence of NDM-1, in multidrug-resistant Enterobacteriaceae in India, Pakistan, and the UK is investigated, and co-ordinated international surveillance is needed.
Journal ArticleDOI
Characterization of a New Metallo-β-Lactamase Gene, blaNDM-1, and a Novel Erythromycin Esterase Gene Carried on a Unique Genetic Structure in Klebsiella pneumoniae Sequence Type 14 from India
Dongeun Yong,Mark Toleman,Christian G. Giske,Hyun Sun Cho,Kristina Sundman,Kyungwon Lee,Timothy R. Walsh +6 more
TL;DR: A Swedish patient of Indian origin traveled to New Delhi, India, and acquired a urinary tract infection caused by a carbapenem-resistant Klebsiella pneumoniae strain that typed to the sequence type 14 complex, showing broad resistance carried on these plasmids.
Journal ArticleDOI
The real threat of Klebsiella pneumoniae carbapenemase-producing bacteria
TL;DR: In this article, the authors reported that the detection of Klebsiella pneumoniae carbapenemases (KPC) producing bacteria may be difficult based on routine antibiotic susceptibility testing.
Journal ArticleDOI
Metallo-β-Lactamases: the Quiet before the Storm?
TL;DR: Their rapid dissemination is worrisome and necessitates the implementation of not just surveillance studies but also metallo-β-lactamase inhibitor studies securing the longevity of important anti-infectives.
Journal ArticleDOI
Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp.
Neil Woodford,Matthew J. Ellington,Juliana Coelho,Jane F. Turton,M. Elaina Ward,Susan Brown,Sebastian G. B. Amyes,David M. Livermore +7 more
TL;DR: A novel multiplex polymerase chain reaction (PCR) assay able to detect and distinguish alleles encoding three subgroups of acquired OXA carbapenemases that are scattered in Acinetobacter spp.
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