N-glycan maturation is crucial for cytokinin-mediated development and cellulose synthesis in Oryza sativa.
Wahyu Indra Duwi Fanata,Kyounghwan Lee,Bo Hwa Son,Jae Yong Yoo,Rikno Harmoko,Ki Seong Ko,Nirmal Kumar Ramasamy,Kyung Hwa Kim,Doo-Byoung Oh,Hyun Suk Jung,Jae-Yean Kim,Sang Yeol Lee,Kyun Oh Lee +12 more
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TLDR
The results strongly support the proposed involvement of N-glycan maturation in transport as well as in the function of membrane proteins that are synthesized via the endomembrane system.Abstract:
Summary
To explore the physiological significance of N-glycan maturation in the plant Golgi apparatus, gnt1, a mutant with loss of N-acetylglucosaminyltransferase I (GnTI) function, was isolated in Oryza sativa. gnt1 exhibited complete inhibition of N-glycan maturation and accumulated high-mannose N-glycans. Phenotypic analyses revealed that gnt1 shows defective post-seedling development and incomplete cell wall biosynthesis, leading to symptoms such as failure in tiller formation, brittle leaves, reduced cell wall thickness, and decreased cellulose content. The developmental defects of gnt1 ultimately resulted in early lethality without transition to the reproductive stage. However, callus induced from gnt1 seeds could be maintained for periods, although it exhibited a low proliferation rate, small size, and hypersensitivity to salt stress. Shoot regeneration and dark-induced leaf senescence assays indicated that the loss of GnTI function results in reduced sensitivity to cytokinin in rice. Reduced expression of A-type O. sativa response regulators that are rapidly induced by cytokinins in gnt1 confirmed that cytokinin signaling is impaired in the mutant. These results strongly support the proposed involvement of N-glycan maturation in transport as well as in the function of membrane proteins that are synthesized via the endomembrane system.read more
Citations
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Plant protein glycosylation
TL;DR: This review focuses on the biosynthesis and function of different protein N-linked glycans in plants, with special emphasis on glycan-mediated quality control processes in the ER and on the biological role of characteristic complex N-glycan structures.
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Cell wall O-glycoproteins and N-glycoproteins: aspects of biosynthesis and function.
Eric Nguema-Ona,Maïté Vicré-Gibouin,Maxime Gotté,Barbara Plancot,Patrice Lerouge,Muriel Bardor,Azeddine Driouich +6 more
TL;DR: The biosynthesis of O-glycoproteins and functional aspects are presented and discussed in this review and plant complex-type N-glycans of proteins are essential for their stability and function.
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Biological significance of complex N-glycans in plants and their impact on plant physiology.
TL;DR: This short review focuses on important recent developments in N-glycan biosynthesis and processing and discusses their implications for future research in plant glycobiology and plant biotechnology.
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The impact of abiotic factors on cellulose synthesis
TL;DR: The impact of four abiotic factors (osmotic conditions, ionic stress, light, and temperature) on the synthesis of cellulose, an important component of the plant cell wall, is reviewed.
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Function of N-glycosylation in plants.
TL;DR: Genetic studies revealed the importance of complex N-glycans in cellulose biosynthesis and growth under stress in plants, and promoted higher capacity of proper protein folding and degradation of misfolded proteins to clear the ER.
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