Showing papers in "FEBS Letters in 2004"

TL;DR: An analysis of the physiological function of the identified genes indicates that the RP approach is powerful for identifying biologically relevant expression changes and can lead to a sharp reduction in the number of replicate experiments needed to obtain reproducible results.

TL;DR: Pannexin 1 channels have a wide expression spectrum, they are of large conductance and permeant for ATP, and they are mechanosensitive, Hence, pannexins are candidates for the release of ATP to the extracellular space upon mechanical stress.

TL;DR: It is shown that hypoxia regulates both expression and activity of CA IX in order to enhance the extracellular acidification, which may have important implications for tumor progression.

TL;DR: It is demonstrated that PD‐1 modulation of T‐cell function involves inhibition of TCR‐mediated phosphorylation of ZAP70 and association with CD3ζ andPD‐1 signaling attenuates PKCθ activation loop phosphorylated in a cognate TCR signal.

TL;DR: Current knowledge on various Hsp90 isoforms regarding their genomic location, molecular evolution, functional differences, differential induction after various environmental stresses and in pathological conditions as well as the growing importance of discriminating between Hsp 90 isoforms in clinical practice are summarized.

TL;DR: Results delineate post‐translation processing of Klotho and possible regulatory mechanisms for secretion ofklotho in vivo and suggest the secreted as well as the membrane‐bound Klotha proteins were suggested to form oligomerized complex.

TL;DR: It is demonstrated that AtHMA4 is localized at the plasma membrane and expressed in tissues surrounding the root vascular vessels and plays a role in metal loading in the xylem.

TL;DR: Thiol‐containing siRNAs corresponding to luciferase, or green fluorescent protein (GFP) transgenes, were synthesized and conjugated to penetratin or transportan via a disulfide bond that is labile in the reducing environment of the cytoplasm.

TL;DR: A mechanism is proposed that links reactive oxygen species (ROS) generation with lipid oxidation processes, ultimately resulting in the formation of similar, highly active signalling compounds in plants.

TL;DR: The emphasis here is on isolated mitochondria and on general mitochondrial properties in order to focus on how mitochondria alone may function to fulfill their physiological roles even though the interactions of mitochondria with other organelles, particularly with endoplasmic and sarcoplasmsic reticulum, may also influence this story.

TL;DR: The FRU gene is a mediator in induction of iron mobilization responses in Arabidopsis, indicating that regulation of iron uptake is conserved in dicot species.

TL;DR: It is shown that Pim‐1 colocalizes and physically interacts with the pro‐apoptotic Bad protein and phosphorylates it in vitro on serine 112, which is a gatekeeper site for its inactivation.

TL;DR: Homology modeling of TaTIP2;1 in combination with site directed mutagenesis suggested a new subgroup of NH3‐transporting aquaporins here called aquaammoniaporins, which complemented NH4 + transport deficiency in yeast.

TL;DR: The human ABCG2 (ABCP/MXR/BCRP) protein is a recently recognized ABC half‐transporter, which forms homodimers in the plasma membrane and actively extrudes a wide variety of chemically unrelated compounds from the cells.

TL;DR: The genetic modification of the N‐glycosylation pathway in Arabidopsis thaliana plants is reported, which represents a limitation for the therapeutic use of recombinant mammalian glycoproteins produced in transgenic plants.

TL;DR: Combined treatment of ATRA or G‐CSF promoted lung regeneration and increased bone marrow‐derived cell numbers in alveoli, which indicated that BMC mobilization might be important in lung regeneration.

TL;DR: Fibril formation of islet amyloid polypeptide (IAPP) is associated with cell death of the insulin‐producing pancreatic β‐cells in patients with Type 2 Diabetes Mellitus and it is shown by fluorescence confocal microscopy on lipid vesicles that the process of hIAPP is accompanied by a loss of barrier function.

TL;DR: The observed effects on lipid binding and filament assembly may explain the pathogenic nature of the E46K mutation in α‐synuclein.

TL;DR: This review summarises the existing data on the molecular effects of microcystin‐LR inhibition of PP1 and PP2A both in vivo and in vitro, and where possible, compares this to the action of okadaic acid.

TL;DR: Results show that at least two peptides, AM and AM2, comprise an adrenomedullin family in mammals, and that AM2 may play pivotal roles in cardiovascular and body fluid regulation.

TL;DR: The high level expression of VLPs with correct S protein conformation by a single recombinant baculovirus offers a potential candidate vaccine for SARS, demonstrating that the VLLP are morphological mimics of virion particles.

TL;DR: Functional studies, especially involving blocking of MMP activities, along with the identification of M MP substrates in the brain strongly suggest that this enzymatic system plays an important physiological role in adult brain neurons, possibly being pivotal for neuronal plasticity.

TL;DR: The preference and potency of the bpVs towards PTEN was validated in vivo as demonstrated and the ability to rescue Ly294002‐induced phosphoinositide 3‐kinase inhibition was demonstrated.

TL;DR: A semi‐empirical molecular model for the rhodopsin paracrystal is presented validating the previously reported results and comparing the model with other currently available models describing the supramolecular structure of GPCRs in the membrane.

TL;DR: A relative quantitative RT‐PCR protocol is developed that allows use of a single internal standard over a wide range of target gene expression levels and suggests that each target sequence possesses an inherent degree of susceptibility to dsRNA‐mediated degradation.

TL;DR: Data from fluorescence‐activated cell sorting and real‐time quantitative RT‐PCR analysis shows that harvested limbal epithelial cells contain SP cells expressing ABCG2, and indicates that ABCG1 positiveLimbal epithelium cells are putative corneal epithelial stem cells.

TL;DR: It is shown that the two‐stage genetic network constructed in a cell‐free expression system is functional within liposomes.

TL;DR: Functional analysis in intact cells revealed fivefold stimulation of 11β‐HSD1 oxoreductase activity and sixfold decrease of dehydrogenase activity upon coexpression with H6PDH, without changing kinetic parameters in cell lysates.

TL;DR: The structural characteristics of the three nuclear phosphoproteins of the high mobility group A family are outlined and related to their participation in chromatin structure alteration in many biological processes such as gene expression, neoplastic transformation, differentiation, and apoptosis are outlined.

TL;DR: The aromatic diamine JSH‐23 compound exhibited inhibitory effect with an IC50 value of 7.1 μM on nuclear factor (NF)‐κB transcriptional activity in lipopolysaccharide (LPS)‐stimulated macrophages RAW 264.7, and interfered LPS‐induced nuclear translocation of NF‐κBs without affecting IκB degradation.