Nanotechnology-Based Cisplatin Intracellular Delivery to Enhance Chemo-Sensitivity of Ovarian Cancer.
Barbara Bortot,Maurizio Mongiat,Erica Valencic,Simeone Dal Monego,Danilo Licastro,Matteo Crosera,Gianpiero Adami,Enrico Rampazzo,Giuseppe Ricci,Federico Romano,Giovanni Maria Severini,Stefania Biffi +11 more
TLDR
It is found that Cis-NP treatment in vivo can significantly reduce tumor burden and aggressiveness compared to the free drug and contribute to improving the drawbacks of conventional intraperitoneal therapy, and therefore will require further investigations in vivo.Abstract:
Background Platinum resistance is a major challenge in the management of ovarian cancer Even low levels of acquired resistance at the cellular level lead to impaired response to cisplatin In ovarian cancer intraperitoneal therapy, nanoparticle formulation can improve the cisplatin's pharmacokinetics and safety profile Purpose This work aimed to investigate the chemo-sensitivity of ovarian cancer SKOV3 cells upon short-term (72h) single treatment of cisplatin and cisplatin-loaded biodegradable nanoparticles (Cis-NP) The aim was then to determine the therapeutic properties of Cis-NP in vivo using a SKOV3-luc cells' xenograft model in mice Methods Cell cytotoxicity was assessed after the exposure of the cell culture to cisplatin or Cis-NP The effect of treatments on EMT and CSC-like phenotype was studied by analyzing a panel of markers by flow cytometry Intracellular platinum concentration was determined by inductively coupled plasma mass spectrometry (ICS-MS), and gene expression was evaluated by RNAseq analysis The efficacy of intraperitoneal chemotherapy was evaluated in a SKOV3-luc cells' xenograft model in mice, through a combination of bioluminescence imaging, histological, and immunohistochemical analyses Results We observed in vitro that short-term treatment of cisplatin has a critical role in determining the potential induction of chemoresistance, and a nanotechnology-based drug delivery system can modulate it The RNAseq analysis underlines a protective effect of nanoparticle system according to their ability to down-regulate several genes involved in chemoresistance, cell proliferation, and apoptosis The highest intracellular platinum concentration obtained with Cis-NP treatment significantly improved the efficacy Consistent with in vitro results, we found that Cis-NP treatment in vivo can significantly reduce tumor burden and aggressiveness compared to the free drug Conclusion Nanoparticle-mediated cisplatin delivery may serve as an intracellular depot impacting the cisplatin pharmacodynamic performance at cellular levels These features may contribute to improving the drawbacks of conventional intraperitoneal therapy, and therefore will require further investigations in vivoread more
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Advanced photodynamic therapy with an engineered M13 phage targeting EGFR: Mitochondrial localization and autophagy induction in ovarian cancer cell lines
TL;DR: In this article , a bacteriophage-based receptor targeted photodynamic therapy for EGFR-positive ovarian cancer was proposed, which showed significant downregulation in the expression of EGFR in cells exposed to M13r-Ce6 after PDT.
Journal ArticleDOI
Advanced photodynamic therapy with an engineered M13 phage targeting EGFR: Mitochondrial localization and autophagy induction in ovarian cancer cell lines
Barbara Bortot,Matteo Calvaresi,Maura Apollonio,Gabriele Baj,Laura Andolfi,Luisa Zupin,Sergio Crovella,Matteo Di Giosia,Andrea Cantelli,Roberto Saporetti,Luca Ulfo,Annapaola Petrosino,Giovanni Di Lorenzo,Federico Romano,Giuseppe Ricci,Maurizio Mongiat,Alberto Danielli,Matteo Calvaresi,Stefania Biffi +18 more
TL;DR: In this paper, a bacteriophage-based receptor targeted photodynamic therapy for EGFR-positive ovarian cancer was proposed, which showed significant downregulation in the expression of EGFR in cells exposed to M13r-Ce6 after PDT.
Journal ArticleDOI
Nanotherapeutic approach to tackle chemotherapeutic resistance of cancer stem cells.
Bruna Stefane Alves de Oliveira,Ana Carolina Correa de Assis,Natália Melo Souza,Luiz Fernando Romanholo Ferreira,Renato N. Soriano,Muhammad Bilal,Hafiz M.N. Iqbal +6 more
TL;DR: In this paper, a review of the most relevant studies that have shown the effects of nanotherapeutics in cancer treatment is presented. And although the studies analyzed are mostly preclinical, they believe that there is strong evidence that nanoparticles can increase the chances of a better prognosis to cancer in the future.
Journal ArticleDOI
Revisiting Antibody-Drug Conjugates and their Predictive Biomarkers in Platinum-Resistant Ovarian Cancer.
TL;DR: In this article, the evidence from clinical trials supporting the development of antibody-drug conjugates targeting folate receptor alpha, sodium-dependent phosphate transporter 2B, dipeptidase 3, mesothelin, mucin 16, and tissue factor using various cytotoxic payloads in ovarian cancer was reviewed.
Journal ArticleDOI
Encapsulation of Alpha-Lipoic Acid in Functional Hybrid Liposomes: Promising Tool for the Reduction of Cisplatin-Induced Ototoxicity
Manuela Curcio,Giuseppe Cirillo,Rosario Amato,Lorenzo Guidotti,Diana Amantea,Michele De Luca,Fiore Pasquale Nicoletta,Francesca Iemma,Mercedes Garcia-Gil +8 more
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