Journal ArticleDOI
Nitrite uptake system in photosynthetic bacterium Rhodopseudomonas capsulata E1F1
TLDR
Nitrate completely suppressed nitrite uptake, whereas ammonia or l -methionine- dl -sulfoximine, which immediately stopped nitrate entrance, only inhibited partially nitrite consumption and it is concluded that in Rps.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1986-01-28. It has received 22 citations till now. The article focuses on the topics: Nitrite transport & Nitrite reductase.read more
Citations
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Journal ArticleDOI
Regulation of nap Gene Expression and Periplasmic Nitrate Reductase Activity in the Phototrophic Bacterium Rhodobacter sphaeroides DSM158
TL;DR: Results indicate that nitrate reduction in R. sphaeroides is mainly regulated at the level of enzyme activity by both nitrate and electron supply and confirm that the Nap system is involved in redox balancing using nitrate as an ancillary oxidant to dissipate excess reductant.
Journal ArticleDOI
3-Nitroadipate, a Metabolic Intermediate for Mineralization of 2,4-Dinitrophenol by a New Strain of a Rhodococcus Species
Rafael Blasco,Edward R. B. Moore,Victor Wray,Dietmar H. Pieper,Kenneth N. Timmis,Francisco Castillo +5 more
TL;DR: The bacterial strain RB1 has been isolated by enrichment cultivation with 2,4-dinitrophenol as the sole nitrogen, carbon, and energy source and characterized, on the basis of 16S rRNA gene sequence comparison, as a Rhodococcus species closely related toRhodococcus opacus.
Journal ArticleDOI
Light-dependent degradation of nitrophenols by the phototrophic bacterium Rhodobacter capsulatus E1F1.
Rafael Blasco,Francisco Castillo +1 more
TL;DR: Rhodobacter capsulatus E1F1, a phototrophic purple nonsulfur bacterium capable of photoassimilating nitrate or nitrite, grew phototrophically in the presence of mono- and dinitrophenols with acetate as a carbon source, the highest growth levels being obtained under microaerobic conditions as mentioned in this paper.
Journal ArticleDOI
Arginine catabolism in the phototrophic bacterium Rhodobacter capsulatus E1F1. Purification and properties of arginase.
TL;DR: The phototrophic bacterium Rhodobacter capsulatus E1F1 grew with L-arginine or L-homo arginine as nitrogen source under light/anaerobiosis but exhibited weak growth levels and the excess of nitrogen was excreted to the medium as ammonia when L-aurine was used as the only source of both carbon and nitrogen.
Journal ArticleDOI
The assimilatory nitrate reduction system of the phototrophic bacterium Rhodobacter capsulatus E1F1
C. Pino,F. Olmo-Mira,Purificación Cabello,Manuel Martínez-Luque,Francisco Castillo,María Dolores Roldán,Conrado Moreno-Vivián +6 more
TL;DR: A 17 kb DNA region encoding the nitrate assimilation (nas) system of this bacterium has been cloned and sequenced and the sequence analysis of these genes and the biochemical properties of the purified nitrate, nitrite and hydroxylamine reductases are reviewed.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Characterization of Rhodopseudomonas capsulata.
TL;DR: Thirty-three strains of Rhodopseudomonas capsulata have been studied in order to develop a more comprehensive characterization of the species, and the characteristics of an “ideal biotype” have been defined, which can be used to distinguish Rps.
Journal ArticleDOI
The determination of nitrate in soil solutions by ultraviolet spectrophotometry
TL;DR: In this article, ultraviolet spectrophotometric analysis has been applied to the determination of nitrate nitrogen over the 0·5 to 10 p.m. range in soil solutions and perfusates in the absence of organic matter.
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The assimilation of nitrate and ammonium by chlamydomonas reinhardi
Ann Thacker,P. J. Syrett +1 more
TL;DR: Experimental evidence indicated that products of ammonium assimilation, rather than ammonium itself, were inhibiting, and it is suggested that light promoted nitrogen assimilation by photophosphorylation.
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Ferredoxin-nitrite reductase from spinach.
TL;DR: Nitrite reductase from a crude homogenate of spinach leaves has been purified about 500-fold and freed from NADP-reductase, and a method based on the reduction of nitrite by ferredoxin chemically reduced with hydrosulfite has been successfully applied to the aerobic assay of the enzyme.