Journal ArticleDOI
Non-reactivity of the selenoenzyme glutathione peroxidase with enzymatically hydroperoxidized phospholipids
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TLDR
The result implies that the membrane-protective function of glutathione peroxidase includes preceeding phospholipase action and excludes a direct interaction of this enzyme with membrane-bound lipid hydroperoxides.Abstract:
Selenium-containing glutathione peroxidase (EC 1.11.1.9) was purified 6000-fold from bovine red blood cells to apparent homogeneity. Lipoxygenase (EC 1.13.11.12) was enriched 20-fold from soybean acetone powder. Linoleic acid was peroxidized with lipoxygenase and then used as a substrate in the glutathione peroxidase reaction. Analogous experiments were conducted with synthetic 1,2-dilinoleoyl-l-α-glycerophosphocholine and with natural bovine heart cardiolipin. The peroxidized phospholipids were reactive with glutathione peroxidase only after enzymatic attack by phospholipase A2 (EC 3.1.1.4). This result implies that the membrane-protective function of glutathione peroxidase includes preceeding phospholipase action and excludes a direct interaction of this enzyme with membrane-bound lipid hydroperoxides.read more
Citations
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Journal ArticleDOI
Ferrous ion oxidation in the presence of xylenol orange for detection of lipid hydroperoxide in low density lipoprotein.
TL;DR: The assay has been validated in the study of lipid peroxidation of low density lipoprotein and phosphatidyl choline liposomes and compared with the ferrous oxidation-xylenol orange assay.
Journal ArticleDOI
Lipid hydroperoxide generation, turnover, and effector action in biological systems
TL;DR: An aspect of related interest that is under intensive investigation is lipid peroxidation/LOOH-mediated stress signaling, which may evoke a variety of cellular responses, ranging from induction of antioxidant enzymes to apoptotic death.
Journal ArticleDOI
The selenoenzyme phospholipid hydroperoxide glutathione peroxidase.
TL;DR: The similarity of amino acid composition, Se content and kinetic mechanism, relative to the difference in substrate specificity, indicates that the two enzymes 'classical' glutathione peroxidase and phospholipid hydroperoxide glutathions are in some way related.
Journal ArticleDOI
The glutathione peroxidases.
TL;DR: It is clear that the selenoperoxidases are involved in cell antioxidant systems, however, they also have more subtle functions in ensuring the regulation and formation of arachadonic acid metabolites that are derived from hydroperoxide intermediates.
Journal ArticleDOI
Biological significance of phospholipid hydroperoxide glutathione peroxidase (PHGPx, GPx4) in mammalian cells.
Hirotaka Imai,Yasuhito Nakagawa +1 more
TL;DR: This review focuses on the structure and biological functions of PHGPx in mammalian cells, which has become clear that lipid hydroperoxides also have an important function as activators of lipoxygenase and cyclooxygenases, participate in inflammation, and act as signal molecules for apoptotic cell death and receptor-mediated signal transduction at the cellular level.
References
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Journal ArticleDOI
Phosphorus Assay in Column Chromatography
TL;DR: If the highest accuracy was not required, the following manipulations simplified and speeded multiple total phosphorus determinations on the eluates from column chromatographic separations.
Journal ArticleDOI
Glutathione peroxidase activity in selenium-deficient rat liver☆
TL;DR: Two peaks of glutathione peroxidase activity were present in the Sephadex G-150 gel filtration chromatogram of rat liver supernatant when 1.5 mM cumene hydroperoxide was used as substrate, and the second peak represents a second glutathienase activity which catalyzes the destruction of organic hydroperoxides but has little activity toward H 2 O 2 and which persists in severe selenium deficiency.
Book ChapterDOI
[44] Glutathione peroxidase
TL;DR: This chapter presents a procedure for the preparation of glutathione peroxidase, which is regarded as a major protective system against endogenously and exogenously induced lipid peroxidation.
Journal ArticleDOI
A simple, specific spray for the detection of phospholipids on thin-layer chromatograms.
John C. Dittmer,Robert L. Lester +1 more
TL;DR: A chemical reagent spray specific for phosphate esters, based on molybdenum blue, gives an instantaneous, specific reaction with phospholipids on silica gel or alumina thin layer chromatography (TLC) plates.