Journal ArticleDOI
Novel endothelium-derived relaxing factors. Identification of factors and cellular targets.
Hong Ding,Chris R. Triggle +1 more
TLDR
In this paper, the authors describe a number of techniques that are being used to identify EDHF and present data that address the contribution of a small increase in extracellular K(+) as an EDHF.About:
This article is published in Journal of Pharmacological and Toxicological Methods.The article was published on 2000-09-01. It has received 24 citations till now. The article focuses on the topics: Endothelium-derived hyperpolarizing factor & Nitroarginine.read more
Citations
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Journal ArticleDOI
Vascular smooth muscle and nitric oxide synthase
Igor B. Buchwalow,Thomas Podzuweit,Werner Böcker,Vera Samoilova,Sylvia Thomas,Maren Wellner,Hideo A. Baba,Horst Robenek,Jürgen Schnekenburger,Markus M. Lerch +9 more
TL;DR: A novel supersensitive immunocytochemical technique of signal amplification with tyramide and electron microscopic immunogold labeling complemented with Western blotting provides the first evidence that, in contrast to the currently accepted view, VSMC in various blood vessels express all three NOS isoforms depending on the blood vessel type.
Journal ArticleDOI
Endothelial cell dysfunction and the vascular complications associated with type 2 diabetes: assessing the health of the endothelium.
Hong Ding,Chris R. Triggle +1 more
TL;DR: The cellular basis for endothelial dysfunction is explored in an attempt to identify appropriate new targets and strategies for the treatment of diabetes and the question of biomarkers for vascular disease is considered and their usefulness for the early detection of and their role as contributors to vascular dysfunction is evaluated.
Journal ArticleDOI
Hydrogen peroxide acts as both vasodilator and vasoconstrictor in the control of perfused mouse mesenteric resistance arteries.
TL;DR: These findings provide the first evidence showing that the same and low concentrations of H2O2 can act as a relaxing factor but also as a vasoconstrictor under conditions in which hyperpolarization is compromised.
Journal ArticleDOI
Role of SKCa and IKCa in endothelium-dependent hyperpolarizations of the guinea-pig isolated carotid artery
Pascale Gluais,Gillian Edwards,Arthur H. Weston,John R. Falck,Paul M. Vanhoutte,Michel Félétou +5 more
TL;DR: This investigation shows that the production of a cytochrome P450 metabolite, and the subsequent activation of BKCa, is unlikely to contribute to the EDHF‐mediated responses in the guinea‐pig carotid artery.
Journal ArticleDOI
Role of endothelium-derived hyperpolarizing factor in endothelial dysfunction during diabetes
TL;DR: Inhibition of endothelium‐dependent relaxation features prominently in a range of cardiovascular diseases, including hypertension, coronary artery disease and diabetes.
References
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Journal ArticleDOI
The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine
TL;DR: It is demonstrated that relaxation of isolated preparations of rabbit thoracic aorta and other blood vessels by ACh requires the presence of endothelial cells, and that ACh, acting on muscarinic receptors of these cells, stimulates release of a substance(s) that causes relaxation of the vascular smooth muscle.
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Physiological roles and properties of potassium channels in arterial smooth muscle
Mark T. Nelson,J. M. Quayle +1 more
TL;DR: The main conclusions of this review are: 1) regulation of arterial smooth muscle membrane potential through activation or inhibition of K+ channel activity provides an important mechanism to dilate or constrict arteries; 2) KV, KCa, KIR, and KATP channels serve unique functions in the regulation of artery membrane potential
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Contractile Properties of Small Arterial Resistance Vessels in Spontaneously Hypertensive and Normotensive Rats
TL;DR: The direct measurement of the intrinsic mechanical and contractile properites of two categories of small arterial resistance vessels in the mesenteric bed of 5-month-old normotensive Wistar-Kyoto rats and spontaneously hypertensive rats suggests the disturbance to the cardiovascular regulatory system which results in hypertension produces similar cellular responses in both the myocardium and the peripheral vasculature.
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Hypertension in mice lacking the gene for endothelial nitric oxide synthase
Paul L. Huang,Zhihong Huang,Hiroshi Mashimo,Kenneth D. Bloch,Michael A. Moskowitz,John A. Bevan,Mark C. Fishman +6 more
TL;DR: Responses to NOS blockade in the mutant mice suggest that non-endothelial isoforms of NOS may be involved in maintaining blood pressure, and eNOS mediates basal vasodilation.
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Role of endothelium-derived nitric oxide in the regulation of blood pressure
TL;DR: Results indicate that nitric oxide formation from L-arginine by the vascular endothelium plays a role in the regulation of blood pressure and in the hypotensive actions of acetylcholine.