Novel single chain cAMP sensors for receptor-induced signal propagation.
TLDR
The development of novel fluorescent indicators for cAMP based on the cAMP-binding domains of Epac and PKA, comprised of only a single binding site lacking cooperativity, catalytic properties, and interactions with other proteins, allow us to easily image free intracellular cAMP and rapid signaling events.About:
This article is published in Journal of Biological Chemistry.The article was published on 2004-09-03 and is currently open access. It has received 703 citations till now. The article focuses on the topics: CREB1 & Yellow fluorescent protein.read more
Citations
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During autophagy mitochondria elongate, are spared from degradation and sustain cell viability
TL;DR: It is shown that mitochondrial morphology determines the cellular response to macroautophagy, and regulated changes in mitochondrial morphology determine the fate of the cell during autophagic degradation.
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Fluorescent Proteins and Their Applications in Imaging Living Cells and Tissues
TL;DR: The structure, evolution, and function of GFP-like proteins and their numerous applications for in vivo imaging are focused on, with particular attention to recent techniques.
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β2-Adrenergic Receptor Redistribution in Heart Failure Changes cAMP Compartmentation
Viacheslav O. Nikolaev,Viacheslav O. Nikolaev,Alexey Moshkov,Alexander R. Lyon,Michele Miragoli,Pavel Novák,Pavel Novák,Helen Paur,Martin J. Lohse,Yuri E. Korchev,Sian E. Harding,Julia Gorelik +11 more
TL;DR: In cardiomyocytes from healthy adult rats and mice, spatially confined β2AR-induced cAMP signals are localized exclusively to the deep transverse tubules, whereas functional β1ARs are distributed across the entire cell surface.
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Persistent cAMP-signals triggered by internalized G-protein-coupled receptors.
Davide Calebiro,Viacheslav O. Nikolaev,Maria Cristina Gagliani,Tiziana de Filippis,Christian Dees,Carlo Tacchetti,Luca Persani,Martin J. Lohse +7 more
TL;DR: Real-time monitoring of G-protein-coupled receptor (GPCR) signaling in native cells suggests that the receptor for thyroid stimulating hormone remains active after internalization, challenging the current model for GPCR signaling.
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Sustained cyclic AMP production by parathyroid hormone receptor endocytosis
Sebastien Ferrandon,Timothy N. Feinstein,Marián Castro,Bin Wang,Richard Bouley,John T. Potts,Thomas J. Gardella,Jean-Pierre Vilardaga,Jean-Pierre Vilardaga +8 more
TL;DR: Analyzing the ligand response by measuring the kinetics of activation and deactivation for each individual reaction step along the PTHR signaling cascade found that during the time frame of G protein coupling and cAMP production, PTHrP action was restricted to the cell surface, whereas PTH(1-34) had moved to internalized compartments where it remained associated with the P THR and Galpha(s), potentially as a persistent and active ternary complex.
References
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The Molecular Biology of Memory Storage: A Dialogue Between Genes and Synapses
TL;DR: This book aims to investigate elementary forms of learning and memory at a cellular molecular level—as specific molecular activities within identified nerve cells withinidentified nerve cells.
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A family of cAMP-binding proteins that directly activate Rap1.
Hiroaki Kawasaki,Gregory M. Springett,Naoki Mochizuki,Shinichiro Toki,Mie Nakaya,Michiyuki Matsuda,David E. Housman,Ann M. Graybiel +7 more
TL;DR: The findings suggest the need to reformulate concepts of cAMP-mediated signaling to include direct coupling to Ras superfamily signaling.
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Discrete microdomains with high concentration of cAMP in stimulated rat neonatal cardiac myocytes.
Manuela Zaccolo,Tullio Pozzan +1 more
TL;DR: In neonatal rat cardiac myocytes, β-adrenergic stimulation generates multiple microdomains with increased concentration of cAMP in correspondence with the region of the transverse tubule/junctional sarcoplasmic reticulum membrane, and such gradients specifically activate a subset of protein kinase A molecules anchored in proximity to the T tubule.
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Cyclic nucleotide research — still expanding after half a century
TL;DR: Research into second messengers has provided a framework for understanding transmembrane signal transduction, receptor–effector coupling, protein-kinase cascades and downregulation of drug responsiveness.
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Localized Effects of cAMP Mediated by Distinct Routes of Protein Kinase A
TL;DR: Targeting of PKA and integration of a wide repertoire of proteins involved in signal transduction into complex signal networks further increase the specificity required for the precise regulation of numerous cellular and physiological processes.
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Discrete microdomains with high concentration of cAMP in stimulated rat neonatal cardiac myocytes.
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