Journal ArticleDOI
Optimization and scale-up of 2,3-butanediol production by Bacillus amyloliquefaciens B10-127
TLDR
B batch fermentation shows similar amount of 2,3-BD obtained in lab-scale fermentation, and it is possible to scale up to larger fermentors without major problems.Abstract:
The effects of culture conditions on 2,3-butanediol (2,3-BD) production and its possible scale-up have been studied. A newly isolated Bacillus amyloliquefaciens B10-127, belonged to GRAS microorganisms and showed a remarkable 2,3-BD producing potency, was used for this experiment. Corn steep liquor, soybean meal and ammonium citrate were found to be the key factors in the fermentation according to the results obtained from the Plackett–Burman experimental design. The optimal concentration range of the three factors was examined by the steepest ascent path, and their optimal concentration were further optimized via response surface methodological approach and determined to be 31.9, 22.0 and 5.58 g/l, respectively. The concentration of the obtained 2,3-BD increased significantly with optimized medium (62.7 g/l) when compared with unoptimized medium (45.7 g/l) and the 2,3-BD productivity was about 2.4-fold (The fermentation time was shorten from 72 to 42 h). To observe scale-up effects, batch fermentation was carried out at various working volumes. At a working volume of 20.0 l, the final 2,3-BD concentration and yield were 61.4 and 0.38 g/g at 36 h with a 2,3-BD productivity of 1.71 g/l h. This result shows similar amount of 2,3-BD obtained in lab-scale fermentation, and it is possible to scale up to larger fermentors without major problems.read more
Citations
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Journal ArticleDOI
Strategies for enhancing fermentative production of acetoin: a review.
Zijun Xiao,Jian R. Lu +1 more
TL;DR: A phylogenetic tree is constructed and the relationship between taxonomy and acetoin producing ability is revealed for the first time, which will serve as a useful guide for the screening of competitive acetoin producers.
Journal ArticleDOI
Acetoin production enhanced by manipulating carbon flux in a newly isolated Bacillus amyloliquefaciens.
TL;DR: A new strain, FMME044, exhibited a remarkable ability to synthesize acetoin and was identified as Bacillus amyloliquefaciens, which is a potential industrial strain for acetoin production.
Journal ArticleDOI
Lignocellulosic bio-refinery approach for microbial 2,3-Butanediol production.
TL;DR: An overview of the recent trends and developments in the microbial production of BDO from different lignocellulose biomass is presented.
Journal ArticleDOI
Improved production of 2,3-butanediol in Bacillus amyloliquefaciens by over-expression of glyceraldehyde-3-phosphate dehydrogenase and 2,3-butanediol dehydrogenase.
TL;DR: Co-overexpression of bdh and gapA genes proved an effective method for enhancing 2,3-BD production and inhibiting the accumulation of unwanted by-products (acetoin, lactate and succinate).
Journal ArticleDOI
Metabolic engineering of a novel Klebsiella oxytoca strain for enhanced 2,3-butanediol production
TL;DR: This study isolated Klebsiella oxytoca GSC 12206, a 2,3-BD native hyper-producing and nonpathogenic bacterium, and constructed a mutant deficient in lactic acid formation by disrupting the ldhA gene which encodes lactate dehydrogenase in glucose fermentation.
References
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