Overexpression of microRNA319 impacts leaf morphogenesis and leads to enhanced cold tolerance in rice (Oryza sativa L.).
Chunhua Yang,Dayong Li,D. Mao,Xue Liu,Chengjun Ji,Xiaobing Li,Xianfeng Zhao,Zhukuan Cheng,Caiyan Chen,Lihuang Zhu +9 more
Reads0
Chats0
TLDR
It is demonstrated that miR319 plays important roles in leaf morphogenesis and cold tolerance in rice and genetically down-regulating the expression of either of the two miR 319-targeted genes in RNA interference plants resulted in enhanced cold tolerance after chilling acclimation.Abstract:
MicroRNA319 (miR319) family is one of the conserved microRNA (miRNA) families among diverse plant species. It has been reported that miR319 regulates plant development in dicotyledons, but little is known at present about its functions in monocotyledons. In rice (Oryza sativa L.), the MIR319 gene family comprises two members, Osa-MIR319a and Osa-MIR319b. Here, we report an expression pattern analysis and a functional characterization of the two Osa-MIR319 genes in rice. We found that overexpressing Osa-MIR319a and Osa-MIR319b in rice both resulted in wider leaf blades. Leaves of osa-miR319 overexpression transgenic plants showed an increased number of longitudinal small veins, which probably accounted for the increased leaf blade width. In addition, we observed that overexpressing osa-miR319 led to enhanced cold tolerance (4 °C) after chilling acclimation (12 °C) in transgenic rice seedlings. Notably, under both 4 and 12 °C low temperatures, Osa-MIR319a and Osa-MIR319b were down-regulated while the expression of miR319-targeted genes was induced. Furthermore, genetically down-regulating the expression of either of the two miR319-targeted genes, OsPCF5 and OsPCF8, in RNA interference (RNAi) plants also resulted in enhanced cold tolerance after chilling acclimation. Our findings in this study demonstrate that miR319 plays important roles in leaf morphogenesis and cold tolerance in rice.read more
Citations
More filters
MicroRNAs: Target Recognition and Regulatory Functions
TL;DR: In this article, a review outlines the current understanding of miRNA target recognition in animals and discusses the widespread impact of miRNAs on both the expression and evolution of protein-coding genes.
Journal ArticleDOI
Abiotic stress responses in plants.
TL;DR: In this paper, the molecular mechanisms underlying the responses of plants to abiotic stresses emphasizes their multilevel nature; multiple processes are involved, including sensing, signalling, transcription, transcript processing, translation and post-translational protein modifications.
Journal ArticleDOI
MicroRNA: a new target for improving plant tolerance to abiotic stress
TL;DR: More function and expression studies will be necessary in order to elucidate the common miRNA-mediated regulatory mechanisms that underlie tolerance to different abiotic stresses, and the use of artificial miRNAs, as well as overexpression and knockout/down of both mi RNAs and their targets, will be the best techniques for determining the specific roles of individual miRNAAs in response to environmental stresses.
Journal ArticleDOI
Cold signaling in plants: Insights into mechanisms and regulation
TL;DR: This review summarizes recent progress in characterizing cold-related genes and the pathways that allow transduction of the cold signal in plants, focusing primarily on studies in Arabidopsis thaliana and rice.
Journal ArticleDOI
MicroRNAs As Potential Targets for Abiotic Stress Tolerance in Plants
TL;DR: Recent updates on plant miRNAs, their biogenesis and functions, target prediction and identification, computational tools and databases available, and their roles in abiotic stress-responses and adaptive mechanisms in major crop plants are provided.
References
More filters
Journal ArticleDOI
PLANT COLD ACCLIMATION: Freezing Tolerance Genes and Regulatory Mechanisms
TL;DR: This review of recent advances in determining the nature and function of genes with roles in freezing tolerance and the mechanisms involved in low temperature gene regulation and signal transduction concludes that cold acclimation includes the expression of certain cold-induced genes that function to stabilize membranes against freeze-induced injury.
Plant cold acclimation: Freezing tolerance genes and regulatory mechanisms
TL;DR: A review of recent advances in determining the nature and function of genes with roles in freezing tolerance and the mechanisms involved in low temperature gene regulation and signal transduction are described in this article.
PatentDOI
MicroRNAs in plants
TL;DR: Mutation of an Arabidopsis Dicer homolog, CARPEL FACTORY, prevents the accumulation of miRNAs, showing that similar mechanisms direct miRNA processing in plants and animals.
Journal ArticleDOI
Origin, biogenesis, and activity of plant microRNAs.
TL;DR: Current advances in understanding of the origin, biogenesis, and mode of action of plant miRNAs are discussed and comparisons with their metazoan counterparts are drawn.
Journal ArticleDOI
Novel and Stress-Regulated MicroRNAs and Other Small RNAs from Arabidopsis
Ramanjulu Sunkar,Jian-Kang Zhu +1 more
TL;DR: It is suggested that a large number of miRNAs and other small regulatory RNAs are encoded by the Arabidopsis genome and that some of them may play important roles in plant responses to environmental stresses as well as in development and genome maintenance.
Related Papers (5)
Novel and Stress-Regulated MicroRNAs and Other Small RNAs from Arabidopsis
Ramanjulu Sunkar,Jian-Kang Zhu +1 more