Journal ArticleDOI
Patch clamp technique: Review of the current state of the art and potential contributions from nanoengineering:
Yan Zhao,Samsoon Inayat,D. A. Dikin,Joshua H. Singer,Rodney S. Ruoff,John B. Troy +5 more
- Vol. 222, Iss: 1, pp 1-11
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TLDR
A review of the advances that have been made in the patch clamp technique over the years is provided and where application of nanotechnology might provide significant contributions in the future is considered.Abstract:
The patch clamp technique permits high-resolution recording of the ionic currents flowing through a cell's plasma membrane. In different configurations, this technique has allowed experimenters to ...read more
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Tissue–electronics interfaces: from implantable devices to engineered tissues
Ron Feiner,Tal Dvir +1 more
TL;DR: Tissue properties affecting device integration are described and electronic systems interfacing with organs and engineered tissues are highlighted.
Journal ArticleDOI
Single Cell Electrical Characterization Techniques
TL;DR: A historical review of single cell electrical properties analysis development from classical techniques to recent advances in microfluidic techniques is presented.
Journal ArticleDOI
A Patch-Clamp ASIC for Nanopore-Based DNA Analysis
TL;DR: A fully integrated high-sensitivity patch-clamp system is proposed for single-molecule deoxyribonucleic acid (DNA) analysis using a nanopore sensor and will functionalize single and multiple solid-state nanopores formed in integrated microfluidic devices for advanced DNA analysis, in scientific and diagnostic applications.
Journal ArticleDOI
A Comparison of the Performance and Application Differences Between Manual and Automated Patch-Clamp Techniques
Xiao Yajuan,Liang Xin,Li Zhiyuan +2 more
TL;DR: Improvements in flexibility with regard to cell types and data quality will broaden the applications of the automated patch clamp systems in both academia and industry.
Journal ArticleDOI
Nanotechnologies for the study of the central nervous system
TL;DR: The development of neuroimaging and electrophysiology tools to identify the aetiology and progression of CNS disorders have led to new insights in the understanding of CNS physiology and the development of novel diagnostic modalities for therapeutic intervention.
References
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Journal ArticleDOI
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
TL;DR: The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution, direct membrane patch potential control, and physical isolation of membrane patches.
Journal ArticleDOI
Single-channel currents recorded from membrane of denervated frog muscle fibres
Erwin Neher,Bert Sakmann +1 more
TL;DR: A more sensitive method of conductance measurement is reported, which, in appropriate conditions, reveals discrete changes in conductance that show many of the features that have been postulated for single ionic channels.
Journal ArticleDOI
Muscarinic activation of ionic currents measured by a new whole-cell recording method
Richard Horn,Alain Marty +1 more
TL;DR: Nystatin, applied extracellularly, is shown to cause a rapid and reversible increase of membrane conductance to cations, and dose-response curves for the effect of ACh on Ca-activated K currents are obtained.
Journal ArticleDOI
Low access resistance perforated patch recordings using amphotericin B
TL;DR: These new approaches can provide a substantial increase in versatility and quality for many kinds of electrophysiological measurements and are used to measure single channel currents in a new "outside-out patch" preparation and to measure the resting voltage of epithelial monolayers.
Journal ArticleDOI
Discrete changes of cell membrane capacitance observed under conditions of enhanced secretion in bovine adrenal chromaffin cells.
TL;DR: The size distribution of step-like capacitance changes is well compatible with the idea that steps of capacitance increase reflect individual events of exocytosis of chromaffin granules, whereas steps of the opposite polarity reflect the formation of vesicles or vacuoles by endocytotic processes.
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