Pax6 Is Required for the Multipotent State of Retinal Progenitor Cells
Till Marquardt,Ruth Ashery-Padan,Nicole Andrejewski,Raffaella Scardigli,François Guillemot,Peter Gruss +5 more
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TLDR
It is demonstrated that Pax6 directly controls the transcriptional activation of Retinogenic bHLH factors that bias subsets of RPCs toward the different retinal cell fates, thereby mediating the full retinogenic potential of RPC's.About:
This article is published in Cell.The article was published on 2001-04-06 and is currently open access. It has received 873 citations till now.read more
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Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system
Jean Livet,Tamily A. Weissman,Hyuno Kang,Ju Lu,Robyn A Bennis,Joshua R. Sanes,Jeff W. Lichtman +6 more
TL;DR: Strategies to visualize synaptic circuits by genetically labelling neurons with multiple, distinct colours are presented and may facilitate the analysis of neuronal circuitry on a large scale.
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Electroporation and RNA interference in the rodent retina in vivo and in vitro.
TL;DR: A rapid and convenient electroporation method for both gain- and loss-of-function studies in vivo and in vitro in the rodent retina is reported, which led to photoreceptor phenotypes similar to those of the corresponding knockout mice.
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Toll-like receptor 3 promotes cross-priming to virus-infected cells
Oliver Schulz,Sandra S. Diebold,Margaret Chen,Tanja I. Näslund,Martijn A. Nolte,Lena Alexopoulou,Lena Alexopoulou,Yasu-Taka Azuma,Richard A. Flavell,Peter Liljeström,Caetano Reis e Sousa +10 more
TL;DR: It is shown that murine CD8α+ dendritic cells are activated by double-stranded (ds)RNA present in virally infected cells but absent from uninfected cells, which indicates that TLR3 may have evolved to permit cross-priming of CTLs against viruses that do not directly infect dendedritic cells.
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Conditional control of gene expression in the mouse
TL;DR: Here, I review binary systems used to analyse complex and multi-staged biological processes, such as embryogenesis and cancer, with unprecedented precision and discuss certain studies that exemplify the advantages and limitations of each system.
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Glial cells generate neurons: the role of the transcription factor Pax6
Nico Heins,Paolo Malatesta,Francesco Cecconi,Masato Nakafuku,Kerry L. Tucker,Michael A. Hack,Prisca Chapouton,Yves-Alain Barde,Magdalena Götz +8 more
TL;DR: An important role of Pax6 as intrinsic fate determinant of the neurogenic potential of glial cells is demonstrated and retrovirally mediated Pax6 expression instructs neurogenesis even in astrocytes from postnatal cortex in vitro.
References
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Induction of ectopic eyes by targeted expression of the eyeless gene in Drosophila
TL;DR: By targeted expression of the ey complementary DNA in various imaginal disc primordia of Drosophila, ectopic eye structures were induced on the wings, the legs, and on the antennae and support the proposition that ey is the master control gene for eye morphogenesis.
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A common progenitor for neurons and glia persists in rat retina late in development
TL;DR: Retrovirus-mediated gene transfer was used to mark cell lineages in vivo in the postnatal rat retina, indicating that a single retinal progenitor can generate remarkably diverse cell types near the end of development.
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Pax-6, a murine paired box gene, is expressed in the developing CNS.
Claudia Walther,Peter Gruss +1 more
TL;DR: The expression pattern of Pax-6 during embryogenesis and the isolation of cDNA clones spanning the entire coding region are described and, in addition to the paired domain, a paired-type homeodomain is contained.
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Subregion- and Cell Type–Restricted Gene Knockout in Mouse Brain
Joe Z. Tsien,Dong Feng Chen,David J. Gerber,Cindy Tom,Eric H. Mercer,David J. Anderson,Mark Mayford,Eric R. Kandel,Susumu Tonegawa +8 more
TL;DR: The method to create mice in which the deletion of virtually any gene of interest is restricted to a subregion or a specific cell type in the brain such as the pyramidal cells of the hippocampal CA1 region should allow a more precise analysis of the impact of a gene mutation on animal behaviors.
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Induction of neurogenesis in the neocortex of adult mice.
Sanjay S. Magavi,Blair R. Leavitt,Blair R. Leavitt,Blair R. Leavitt,Jeffrey D. Macklis,Jeffrey D. Macklis +5 more
TL;DR: It is shown that endogenous neural precursors can be induced in situ to differentiate into mature neurons, in regions of adult mammalian neocortex that do not normally undergo any neurogenesis.