Peroxygenases en route to becoming dream catalysts. What are the opportunities and challenges
TLDR
Peroxygenases are promising catalysts for preparative oxyfunctionalization chemistry as they combine the versatility of P450 monooxygenase with simplicity of cofactor-independent enzymes.About:
This article is published in Current Opinion in Chemical Biology.The article was published on 2017-04-01 and is currently open access. It has received 178 citations till now.read more
Citations
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Biocatalytic Oxidation Reactions: A Chemist's Perspective
JiaJia Dong,Elena Fernández-Fueyo,Frank Hollmann,Caroline E. Paul,Milja Pesic,Sandy Schmidt,Yonghua Wang,Sabry H. H. Younes,Wuyuan Zhang +8 more
TL;DR: The most important recent developments in the field of biocatalytic oxidation chemistry are critically summarised and the most pressing bottlenecks as well as promising solutions are identified.
Journal ArticleDOI
Extending the application of biocatalysis to meet the challenges of drug development
Paul N. Devine,Roger M. Howard,Rajesh Kumar,Matthew P. Thompson,Matthew D. Truppo,Matthew D. Truppo,Nicholas J. Turner +6 more
TL;DR: Significant advances in enzyme discovery, high-throughput screening and protein engineering have substantially expanded the available biocatalysts, and consequently, many more synthetic transformations are now possible, leading to greater predictability and confidence when scaling up these processes.
Journal ArticleDOI
Selective aerobic oxidation reactions using a combination of photocatalytic water oxidation and enzymatic oxyfunctionalizations
Wuyuan Zhang,Elena Fernández-Fueyo,Yan Ni,Morten M. C. H. van Schie,Jenö Gacs,Rokus Renirie,Ron Wever,Francesco G. Mutti,Dörte Rother,Miguel Alcalde,Frank Hollmann +10 more
TL;DR: It is shown that visible-light-driven, catalytic water oxidation can be used for in situ generation of H2O2 from water, rendering the peroxygenase catalytically active, allowing efficient oxyfunctionalization without stoichiometric reductants.
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Late-stage C-H functionalization offers new opportunities in drug discovery
TL;DR: In this paper, the authors provide a toolbox for chemists in academia as well as industrial practitioners, and introduce guiding principles for the application of late-stage functionalization strategies to access new molecules of interest.
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Broadening the Scope of Biocatalysis in Sustainable Organic Synthesis
TL;DR: This Review is aimed at synthetic organic chemists who may be familiar with organometallic catalysis but have no experience with biocatalysis, and seeks to provide an answer to the perennial question: if it is so attractive, why wasn't it extensively used in the past?
References
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Tandem-yeast expression system for engineering and producing unspecific peroxygenase.
TL;DR: This work describes a tandem-yeast expression system for UPO engineering and large scale production that ensures the engineering of UPOs to use them in future industrial applications as well as large scaleProduction.
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The Oxygen Dilemma: A Severe Challenge for the Application of Monooxygenases?
Dirk Holtmann,Frank Hollmann +1 more
TL;DR: The oxygen dilemma arises from monooxygenases' dependency on O2 and the undesired uncoupling reaction, and the nature and some promising solutions are discussed.
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Selective hydroxylation of alkanes by an extracellular fungal peroxygenase
Sebastian Peter,Matthias Kinne,Xiaoshi Wang,René Ullrich,Gernot Kayser,John T. Groves,Martin Hofrichter +6 more
TL;DR: Results point to a hydrogen abstraction and oxygen rebound mechanism for alkane hydroxylation and may have a role in the bioconversion of natural and anthropogenic alkane‐containing structures in soils, plant litter, and wood.
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Specific Photobiocatalytic Oxyfunctionalization Reactions
Ekaterina Churakova,Martin Kluge,René Ullrich,Isabel W. C. E. Arends,Martin Hofrichter,Frank Hollmann +5 more
TL;DR: Aae APO is an active and versatile catalyst for enantiospecific hydroxylation and epoxidation reactions and under nonoptimized reaction conditions AaeAPO performance exceeds that reported for CPO by at least two orders of magnitude.
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Visible light-driven and chloroperoxidase-catalyzed oxygenation reactions.
TL;DR: Robust peroxidase-catalyzed enantiospecific oxyfunctionalizations can be achieved by simple light-driven in situ generation of hydrogen peroxide.