Peroxygenases en route to becoming dream catalysts. What are the opportunities and challenges
TLDR
Peroxygenases are promising catalysts for preparative oxyfunctionalization chemistry as they combine the versatility of P450 monooxygenase with simplicity of cofactor-independent enzymes.About:
This article is published in Current Opinion in Chemical Biology.The article was published on 2017-04-01 and is currently open access. It has received 178 citations till now.read more
Citations
More filters
Journal ArticleDOI
Selective Oxygenation of Ionones and Damascones by Fungal Peroxygenases
Esteban D. Babot,Carmen Aranda,José C Del Rı O,René Ullrich,Jan Kiebist,Katrin Scheibner,Martin Hofrichter,Angel T Martı Nez,Ana Gutiérrez +8 more
TL;DR: This work shows the ability of unspecific peroxygenases from several fungi to catalyze the oxyfunctionalization of alpha- and beta-ionones and damascones, yielding oxygenated products such as hydroxy-, oxo-, carboxy- and epoxy-derivatives that are interesting compounds for the flavor and fragrance and pharmaceutical industries.
Journal ArticleDOI
Chemoenzymatic Cascade Conversion of Linoleic Acid into a Secondary Fatty Alcohol Using a Combination of 13S-Lipoxygenase, Chemical Reduction, and a Photo-Activated Decarboxylase
Journal ArticleDOI
Repertoire of Computationally Designed Peroxygenases for Enantiodivergent C–H Oxyfunctionalization Reactions
Patricia Gómez de Santos,Ivan Mateljak,Manh Dat Hoang,Sarel J. Fleishman,Frank Hollmann,Miguel Alcalde +5 more
TL;DR: In this article, the authors applied the FuncLib algorithm based on phylogenetic and Rosetta calculations to design a diverse repertoire of active, stable, and enantiodivergent fungal peroxygenases.
Journal ArticleDOI
Biocatalysis for terpene-based polymers.
TL;DR: The inherent monomer reactivity of norcamphor is unlocked, a bicyclic ketone used as a monoterpene model system in this study, to afford polyesters with unprecedented backbones and the concepts discussed herein are widely applicable for the valorization of terpenes and other cyclic building blocks using chemoenzymatic strategies.
Journal ArticleDOI
Selective oxyfunctionalisation reactions driven by sulfite oxidase‐catalysed in situ generation of H2O2
Morten M. C. H. van Schie,Alexander T. Kaczmarek,Florian Tieves,Patricia Gomez de Santos,Caroline E. Paul,Isabel W. C. E. Arends,Miguel Alcalde,Günter Schwarz,Frank Hollmann +8 more
TL;DR: The application of (modified) sulfite oxidases to couple the oxidation of sulfites to the reduction of oxygen enables us to use calcium sulfite, an industrial waste product from scrubbing flue gases, as an electron donor to reduce oxygen.
References
More filters
Journal ArticleDOI
Engineering the third wave of biocatalysis
Uwe T. Bornscheuer,Gjalt W. Huisman,Romas J. Kazlauskas,Romas J. Kazlauskas,Stefan Lutz,Jeffrey C. Moore,Karen Robins +6 more
TL;DR: Applications of protein-engineered biocatalysts ranging from commodity chemicals to advanced pharmaceutical intermediates that use enzyme catalysis as a key step are discussed.
Journal ArticleDOI
Glucose oxidase--an overview.
TL;DR: Various purification techniques for higher recovery of glucose oxidase are described here, and issues of enzyme kinetics, stability studies and characterization are addressed.
Journal ArticleDOI
Laboratory Evolution of Stereoselective Enzymes: A Prolific Source of Catalysts for Asymmetric Reactions
TL;DR: Emphasis is placed on the development of methods to make laboratory evolution faster and more efficient, thus providing chemists and biotechnologists with a rich and non-ending source of robust and selective catalysts for a variety of useful applications.
Journal ArticleDOI
Cytochrome P450 monooxygenases: an update on perspectives for synthetic application.
Vlada B. Urlacher,Marco Girhard +1 more
TL;DR: A short up-to-date overview of recent results on P450 engineering for technical applications including aspects of whole-cell biocatalysis with engineered recombinant enzymes and recently identified P450s with novel biotechnologically relevant properties are given.
Journal ArticleDOI
Guidelines and Cost Analysis for Catalyst Production in Biocatalytic Processes
TL;DR: It is seen that the fermentation yield in terms of final achievable cell concentration and expression level as well as the production scale are crucial for decreasing the total cost contribution of the biocatalyst.