Peroxygenases en route to becoming dream catalysts. What are the opportunities and challenges
TLDR
Peroxygenases are promising catalysts for preparative oxyfunctionalization chemistry as they combine the versatility of P450 monooxygenase with simplicity of cofactor-independent enzymes.About:
This article is published in Current Opinion in Chemical Biology.The article was published on 2017-04-01 and is currently open access. It has received 178 citations till now.read more
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Peroxide-Mediated Oxygenation of Organic Compounds by Fungal Peroxygenases
Martin Hofrichter,Harald Kellner,Robert Herzog,Alexander Karich,Jan Kiebist,Katrin Scheibner,René Ullrich +6 more
TL;DR: Nearly 20 years of peroxygenase research is reviewed, considering basic mechanistic, molecular, phylogenetic, and biotechnological aspects, including more specific one-electron oxidations.
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Structural and biochemical studies enlighten the unspecific peroxygenase from Hypoxylon sp. EC38 as an efficient oxidative biocatalyst.
Laura Rotilio,Alexander Swoboda,Katharina Ebner,Claudia Rinnofner,Anton Glieder,Wolfgang Kroutil,Andrea Mattevi +6 more
TL;DR: In this article, an unspecific peroxygenase from Hypoxylon sp. EC38 (HspUPO) was identified in an activity-based screen of six putative peroxidegenase enzymes that were heterologously expressed in Pichia pastoris.
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In Situ O2 Generation for Biocatalytic Oxyfunctionalization Reactions
TL;DR: This concept paper discusses strategies to enhance the O2 availability for biocatalytic oxyfunctionalizations with a focus on the in situ generation of O2 from water.
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How To Break the Janus Effect of H 2 O 2 in Biocatalysis? Understanding Inactivation Mechanisms To Generate more Robust Enzymes
TL;DR: It is demonstrated that a rational approach combining multiscale MD simulations and Born–Oppenheimer ab initio QM/MM MD simulations is an efficient approach to rapidly identify improved enzyme variants to improve enzyme resistance against H2O2.
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Nuclear Waste and Biocatalysis: A Sustainable Liaison?
Wuyuan Zhang,Wuyuan Zhang,Huanhuan Liu,Morten M. C. H. van Schie,Peter-Leon Hagedoorn,Miguel Alcalde,Antonia G. Denkova,Kristina Djanashvili,Frank Hollmann +8 more
TL;DR: It is demonstrated that nuclear waste can also be used to drive selective, peroxygenase-catalyzed oxyfunctionalization reactions, challenging the view on nuclear waste in terms of sustainability.
References
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Engineering the third wave of biocatalysis
Uwe T. Bornscheuer,Gjalt W. Huisman,Romas J. Kazlauskas,Romas J. Kazlauskas,Stefan Lutz,Jeffrey C. Moore,Karen Robins +6 more
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Laboratory Evolution of Stereoselective Enzymes: A Prolific Source of Catalysts for Asymmetric Reactions
TL;DR: Emphasis is placed on the development of methods to make laboratory evolution faster and more efficient, thus providing chemists and biotechnologists with a rich and non-ending source of robust and selective catalysts for a variety of useful applications.
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Cytochrome P450 monooxygenases: an update on perspectives for synthetic application.
Vlada B. Urlacher,Marco Girhard +1 more
TL;DR: A short up-to-date overview of recent results on P450 engineering for technical applications including aspects of whole-cell biocatalysis with engineered recombinant enzymes and recently identified P450s with novel biotechnologically relevant properties are given.
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Guidelines and Cost Analysis for Catalyst Production in Biocatalytic Processes
TL;DR: It is seen that the fermentation yield in terms of final achievable cell concentration and expression level as well as the production scale are crucial for decreasing the total cost contribution of the biocatalyst.