Peroxygenases en route to becoming dream catalysts. What are the opportunities and challenges
TLDR
Peroxygenases are promising catalysts for preparative oxyfunctionalization chemistry as they combine the versatility of P450 monooxygenase with simplicity of cofactor-independent enzymes.About:
This article is published in Current Opinion in Chemical Biology.The article was published on 2017-04-01 and is currently open access. It has received 178 citations till now.read more
Citations
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Biocatalytic Oxidation Reactions: A Chemist's Perspective
JiaJia Dong,Elena Fernández-Fueyo,Frank Hollmann,Caroline E. Paul,Milja Pesic,Sandy Schmidt,Yonghua Wang,Sabry H. H. Younes,Wuyuan Zhang +8 more
TL;DR: The most important recent developments in the field of biocatalytic oxidation chemistry are critically summarised and the most pressing bottlenecks as well as promising solutions are identified.
Journal ArticleDOI
Extending the application of biocatalysis to meet the challenges of drug development
Paul N. Devine,Roger M. Howard,Rajesh Kumar,Matthew P. Thompson,Matthew D. Truppo,Matthew D. Truppo,Nicholas J. Turner +6 more
TL;DR: Significant advances in enzyme discovery, high-throughput screening and protein engineering have substantially expanded the available biocatalysts, and consequently, many more synthetic transformations are now possible, leading to greater predictability and confidence when scaling up these processes.
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Selective aerobic oxidation reactions using a combination of photocatalytic water oxidation and enzymatic oxyfunctionalizations
Wuyuan Zhang,Elena Fernández-Fueyo,Yan Ni,Morten M. C. H. van Schie,Jenö Gacs,Rokus Renirie,Ron Wever,Francesco G. Mutti,Dörte Rother,Miguel Alcalde,Frank Hollmann +10 more
TL;DR: It is shown that visible-light-driven, catalytic water oxidation can be used for in situ generation of H2O2 from water, rendering the peroxygenase catalytically active, allowing efficient oxyfunctionalization without stoichiometric reductants.
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Late-stage C-H functionalization offers new opportunities in drug discovery
TL;DR: In this paper, the authors provide a toolbox for chemists in academia as well as industrial practitioners, and introduce guiding principles for the application of late-stage functionalization strategies to access new molecules of interest.
Journal ArticleDOI
Broadening the Scope of Biocatalysis in Sustainable Organic Synthesis
TL;DR: This Review is aimed at synthetic organic chemists who may be familiar with organometallic catalysis but have no experience with biocatalysis, and seeks to provide an answer to the perennial question: if it is so attractive, why wasn't it extensively used in the past?
References
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Suicide inactivation of peroxidases and the challenge of engineering more robust enzymes.
TL;DR: The catalytic cycle of peroxidases and the mechanism of the suicide inactivation process are discussed to establish a broad knowledge base for future rational protein engineering.
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Regio- and stereoselectivity of P450-catalysed hydroxylation of steroids controlled by laboratory evolution
TL;DR: It is demonstrated that directed evolution using iterative saturation mutagenesis provides a means to solve synthetic problems of this kind by preparing mutants that can regio- and stereoselectively oxidize either position.
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Terminal Olefin (1-Alkene) Biosynthesis by a Novel P450 Fatty Acid Decarboxylase from Jeotgalicoccus Species
Mathew Rude,Tarah S. Baron,Shane Brubaker,Murtaza F. Alibhai,Stephen B. del Cardayre,Andreas Schirmer +5 more
TL;DR: A group of bacteria, Jeotgalicoccus spp.
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Tuning P450 Enzymes as Oxidation Catalysts
TL;DR: The development of catalytic systems for the controlled oxidation of C–H bonds remains a highly sought-after goal in chemistry owing to the great utility of such transformation toward expediting the synthesis and functionalization of organic molecules.
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Enzymatic hydroxylation of aromatic compounds
René Ullrich,Martin Hofrichter +1 more
TL;DR: The article deals with Rieske-type and α-keto acid-dependent dioxygenases, as well as different non-heme monooxygenases (di-iron, pterin, and flavin enzymes), tyrosinase, laccase, and hydroxyl radical generating systems.