Journal ArticleDOI
Persistent infection of mouse fibroblasts with coxsackievirus
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TLDR
Infection of fibroblast cell lines initiated from BALB/c or NFR mice with coxsackievirus B3 or B4 resulted in infections which persisted for a limited number of subpassages of the infected cells in most cases, but for over a year in one case.Abstract:
Infection of fibroblast cell lines initiated from BALB/c or NFR mice with coxsackievirus B3 (CBV-3) or B4 (CBV-4) resulted in infections which persisted for a limited number of subpassages of the infected cells in most cases, but for over a year in one case. In all instances primary acute infections were characterized by cytopathology and release of infectious virus progeny. Viral antigen could be detected during the acute phase of infection, but not in subcultured infected cells. Infectious center assays showed that every cell was infected during the acute phase of infection, but that from the first subcultivation on, the numbers of cells which were able to initiate infection were greatly reduced. The long term persistent CBV-3 infection was characterized by wide fluctuations in titers of virus released into the supernatant fluids. Interferon did not appear to play a role in maintenance of the persistent infection. Information derived from studies on mechanisms of CBV persistence in thein vitro model may help to elucidate the role of CBV in chronic human diseases such as myocarditis.read more
Citations
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Journal ArticleDOI
Diabetes mellitus due to viruses - some recent developments
TL;DR: It is clear that there are many viruses with the potential to induce diabetes, and a viral involvement in the pathogenesis of diabetes has been established in some instances.
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Molecular Mechanisms of Coxsackievirus Persistence in Chronic Inflammatory Myopathy: Viral RNA Persists through Formation of a Double-Stranded Complex without Associated Genomic Mutations or Evolution
TL;DR: It is demonstrated that persistence of coxsackievirus RNA in muscle is not facilitated by distinct genetic changes in the virus that give rise to replication-defective forms but occurs primarily through production of stable double-stranded RNA that is produced as the acute viral infection resolves.
Journal ArticleDOI
The Role of B Lymphocytes in Coxsackievirus B3 Infection
Ignacio Mena,Christopher M. Perry,Stephanie Harkins,Fernando Rodriguez,John R. Gebhard,J. Lindsay Whitton +5 more
TL;DR: It is confirmed that Type B coxsackievirus (CVB) infects B lymphocytes, and extended these findings to show that this is a productive infection involving approximately 1 to 10% of the cells; however, infectious center assays show that other splenocytes are infected at approximately the same frequency.
Journal ArticleDOI
Clinical and experimental aspects of viral myocarditis.
TL;DR: In this paper, the role of the immune system in cardiac damage and the likelihood that the infection triggers an autoimmune response to heart-specific antigens is discussed, and evidence supporting various theories of virus-initiated autoimmunity and disease pathogenesis are delineated.
Journal ArticleDOI
Genomic detection of enteroviruses in the myocardium--studies on animal hearts with coxsackievirus B3 myocarditis and endomyocardial biopsies from patients with myocarditis and dilated cardiomyopathy.
TL;DR: A link between viral myocarditis and dilated cardiomyopathy is supported and enteroviral RNA sequences identified by in situ hybridization in myocardial cells and some interstitial cells in and around the myocarditic lesions in animals.
References
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The preparation of 131i-labelled human growth hormone of high specific radioactivity
TL;DR: The loss of immunological reactivity at high specific radioactivities or at high levels of chemical substitution with STAI/sup 127/!iodine is demonstrated.
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Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific Activity
W M Hunter,F. C. Greenwood +1 more
TL;DR: Current procedures for the immunological assay of protein hormones in human plasma require the routine preparation of hormones labelled with iodine-131 of high specific activity, and this work demonstrates the importance of knowing the carrier and removal status of iodine.
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A method of trace iodination of proteins for immunologic studies.
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Rapid evolution of RNA genomes
John J. Holland,Katherine R. Spindler,Katherine R. Spindler,Frank M. Horodyski,Elizabeth A. Grabau,Stuart T. Nichol,Scott VandePol +6 more
TL;DR: RNA viruses show high mutation frequencies partly because of a lack of the proofreading enzymes that assure fidelity of DNA replication, and high rates of replication reflected in rates of RNA genome evolution which can be more than a millionfold greater than the rates of the DNA chromosome evolution of their hosts.