Phospholipase D Stimulates Release of Nascent Secretory Vesicles from the trans-Golgi Network
Ye-Guang Chen,Anirban Siddhanta,Cary D. Austin,Scott M. Hammond,Tsung Chang Sung,Michael A. Frohman,Andrew J. Morris,Dennis Shields +7 more
TLDR
It is demonstrated that immunoaffinity-purified human PLD1 stimulated nascent secretory vesicle budding from the TGN and ARF-1 stimulated endogenous PLD activity in Golgi membranes approximately threefold and this activation correlated with its enhancement of vesicles budding.Abstract:
Phospholipase D (PLD) is a phospholipid hydrolyzing enzyme whose activation has been implicated in mediating signal transduction pathways, cell growth, and membrane trafficking in mammalian cells. Several laboratories have demonstrated that small GTP-binding proteins including ADP-ribosylation factor (ARF) can stimulate PLD activity in vitro and an ARF-activated PLD activity has been found in Golgi membranes. Since ARF-1 has also been shown to enhance release of nascent secretory vesicles from the TGN of endocrine cells, we hypothesized that this reaction occurred via PLD activation. Using a permeabilized cell system derived from growth hormone and prolactin-secreting pituitary GH3 cells, we demonstrate that immunoaffinity-purified human PLD1 stimulated nascent secretory vesicle budding from the TGN approximately twofold. In contrast, a similarly purified but enzymatically inactive mutant form of PLD1, designated Lys898Arg, had no effect on vesicle budding when added to the permeabilized cells. The release of nascent secretory vesicles from the TGN was sensitive to 1% 1-butanol, a concentration that inhibited PLD-catalyzed formation of phosphatidic acid. Furthermore, ARF-1 stimulated endogenous PLD activity in Golgi membranes approximately threefold and this activation correlated with its enhancement of vesicle budding. Our results suggest that ARF regulation of PLD activity plays an important role in the release of nascent secretory vesicles from the TGN.read more
Citations
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Journal ArticleDOI
ADP-ribosylation factor and phosphatidic acid levels in Golgi membranes during budding of coatomer-coated vesicles.
TL;DR: It is suggested that ADP-ribosylation factor, like many other G proteins, is a multifunctional protein with roles in trafficking and phospholipid signaling.
Journal ArticleDOI
Localization and regulation of phospholipase D2 by ARF6.
Masami Hiroyama,John H. Exton +1 more
TL;DR: It is concluded that PLD2 is selectively activated by ARF6 in vivo and that disruption of the actin cytoskeleton does not affect this activation.
Journal ArticleDOI
Phospholipase D1-Promoted Release of Tissue Plasminogen Activator Facilitates Neurite Outgrowth
TL;DR: In this paper, the authors examined potential roles for the signaling enzyme phospholipase D1 (PLD1), which promotes regulated exocytosis in non-CNS cell types, and which previously demonstrated increases in expression in hippocampal neurons during seizure-induced mossy fiber sprouting.
Journal ArticleDOI
Avl9p, a Member of a Novel Protein Superfamily, Functions in the Late Secretory Pathway
Edina Harsay,Randy Schekman +1 more
TL;DR: A novel conserved protein, Avl9p, was identified, the absence of which conferred lethality in a vps1Delta apl2Delta strain background (lacking a dynamin and an adaptor-protein complex 1 subunit), which resulted in secretory defects as well as accumulation of Golgi-like membranes.
Journal ArticleDOI
Similarities in the induction of post-Golgi vesicles by the vaccinia virus F13L protein and phospholipase D.
Matloob Husain,Bernard Moss +1 more
TL;DR: It is shown that the F13L protein has a general effect on the trafficking of integral membrane proteins from the Golgi apparatus, as both the vaccinia virus A36R protein and the vesicular stomatitis virus G protein also colocalized with the F 13L protein in vesicles.
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