Phospholipase D Stimulates Release of Nascent Secretory Vesicles from the trans-Golgi Network
Ye-Guang Chen,Anirban Siddhanta,Cary D. Austin,Scott M. Hammond,Tsung Chang Sung,Michael A. Frohman,Andrew J. Morris,Dennis Shields +7 more
TLDR
It is demonstrated that immunoaffinity-purified human PLD1 stimulated nascent secretory vesicle budding from the TGN and ARF-1 stimulated endogenous PLD activity in Golgi membranes approximately threefold and this activation correlated with its enhancement of vesicles budding.Abstract:
Phospholipase D (PLD) is a phospholipid hydrolyzing enzyme whose activation has been implicated in mediating signal transduction pathways, cell growth, and membrane trafficking in mammalian cells. Several laboratories have demonstrated that small GTP-binding proteins including ADP-ribosylation factor (ARF) can stimulate PLD activity in vitro and an ARF-activated PLD activity has been found in Golgi membranes. Since ARF-1 has also been shown to enhance release of nascent secretory vesicles from the TGN of endocrine cells, we hypothesized that this reaction occurred via PLD activation. Using a permeabilized cell system derived from growth hormone and prolactin-secreting pituitary GH3 cells, we demonstrate that immunoaffinity-purified human PLD1 stimulated nascent secretory vesicle budding from the TGN approximately twofold. In contrast, a similarly purified but enzymatically inactive mutant form of PLD1, designated Lys898Arg, had no effect on vesicle budding when added to the permeabilized cells. The release of nascent secretory vesicles from the TGN was sensitive to 1% 1-butanol, a concentration that inhibited PLD-catalyzed formation of phosphatidic acid. Furthermore, ARF-1 stimulated endogenous PLD activity in Golgi membranes approximately threefold and this activation correlated with its enhancement of vesicle budding. Our results suggest that ARF regulation of PLD activity plays an important role in the release of nascent secretory vesicles from the TGN.read more
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Ral and Rho-dependent activation of phospholipase D in v-Raf-transformed cells.
Paul Frankel,Miguel Ramos,Judith Flom,Sergei Bychenok,Troy Joseph,Eugen Kerkhoff,Ulf R. Rapp,Larry A. Feig,David A. Foster +8 more
TL;DR: A novel mechanism for PLD activation by v-Raf that is independent of PKC, but dependent upon both Ral and Rho GTPases is described.
Journal ArticleDOI
Phosphatidylinositol 5-kinase stimulates apical biosynthetic delivery via an Arp2/3-dependent mechanism
TL;DR: A previously unidentified role for actin comet-mediated propulsion in the biosynthetic delivery of a subset of apical proteins is suggested in membrane transport.
Journal ArticleDOI
Phospholipase D2 functions as a downstream signaling molecule of MAP kinase pathway in L1‐stimulated neurite outgrowth of cerebellar granule neurons
Hiroshi Watanabe,Masakazu Yamazaki,Hideyuki Miyazaki,Hideyuki Miyazaki,Chihiro Arikawa,Kouichi Itoh,Takehiko Sasaki,Tomohiko Maehama,Michael A. Frohman,Yasunori Kanaho +9 more
TL;DR: Results provide evidence that PLD2 functions as a downstream signaling molecule of ERK to mediate the L1‐dependent neurite outgrowth of CGNs, a mechanism that may be related to alcohol‐related neurodevelopmental disorders.
Journal ArticleDOI
Regulation of ADL6 activity by its associated molecular network.
TL;DR: It is demonstrated that ADL6 (Arabidopsis dynamin-like protein 6), due to its domain arrangement, behaves similarly to the animal dynamins and intrinsic GTPase activity is regulated by its association with acidic phospholipids and an SH3 (Src homology 3)-containing protein.
Journal ArticleDOI
Vaccinia Virus F13L Protein with a Conserved Phospholipase Catalytic Motif Induces Colocalization of the B5R Envelope Glycoprotein in Post-Golgi Vesicles
Matloob Husain,Bernard Moss +1 more
TL;DR: It is suggested that the F13L protein, like its human phospholipase D homolog, regulates vesicle formation and that this process is involved in intracellular enveloped virion membrane formation.
References
More filters
Journal Article
Mechanisms of intracellular protein transport
TL;DR: The general protein apparatus used by all eukaryotes for intracellular transport, including secretion and endocytosis, and for triggered exocyTosis of hormones and neurotransmitters, is uncovered.
Journal ArticleDOI
Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting
Peter Schu,Kaoru Takegawa,Michael J. Fry,Jeffrey H. Stack,Michael D. Waterfield,Scott D. Emr +5 more
TL;DR: Overexpression of VPS34p resulted in an increase in PI 3-kinase activity, and this activity was specifically precipitated with antisera to Vps34p.
Journal ArticleDOI
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TL;DR: PC hydrolysis by PLA2, PLC or PLD is a widespread response elicited by most growth factors, cytokines, neurotransmitters, hormones and other extracellular signals and the mechanisms can involve G-proteins, PKC, Ca2+ and tyrosine kinase activities.
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ADP-ribosylation factor, a small GTP-dependent regulatory protein, stimulates phospholipase D activity
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Phosphoinositides as Regulators in Membrane Traffic
TL;DR: Growing evidence suggests that phosphorylation-dephosphorylation of the polar heads of phosphoinositides in specific intracellular locations signals either the recruitment or the activation of proteins essential for vesicular transport.