Journal ArticleDOI
Purification and characterization of isoforms of β-galactosidases in mung bean seedlings
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TLDR
Double immunodiffusion analysis indicated thatbeta-galactosidases I, II, III and V are immunologically similar to each other, while beta-galactsosidase IV shares partially identical antigenic determinants with the other four isoforms.About:
This article is published in Phytochemistry.The article was published on 2001-06-01. It has received 70 citations till now. The article focuses on the topics: Chromatofocusing & Molecular mass.read more
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Primary cell wall metabolism: tracking the careers of wall polymers in living plant cells
TL;DR: It is concluded that some proposed wall enzymes, for example XTHs, do act in vivo, but that for other enzymes this is not proven, and methods are reviewed by which reactions involving structural wall polysaccharides can be tracked in vivo.
Journal ArticleDOI
β Galactosidases and their potential applications: a review
TL;DR: Immobilized β galactosidases are employed for the continuous hydrolysis of lactose from whey and milk in a number of reactors such as hollow fiber reactors, tapered column reactors, packed bed reactors, fluidized bed reactors etc.
Journal ArticleDOI
Plant glycoside hydrolases involved in cell wall polysaccharide degradation.
Zoran Minic,Lise Jouanin +1 more
TL;DR: Current knowledge about plant glycoside hydrolases that participate in the degradation and reorganisation of cell wall polysaccharides in plants focussing particularly on those from Arabidopsis thaliana are summarized.
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Proteomic Analysis of Seed Dormancy in Arabidopsis
TL;DR: Comparative studies carried out with freshly harvested dormant and after-ripened non-dormant seeds revealed a specific differential accumulation of 32 proteins, which suggested that proteins associated with metabolic functions potentially involved in germination can accumulate duringAfter-ripening in the dry state leading to dormancy release.
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The SENSITIVE TO FREEZING2 Gene, Required for Freezing Tolerance in Arabidopsis thaliana, Encodes a β-Glucosidase
TL;DR: After purification from a heterologous expression system, AtSFR2 displayed a specific hydrolytic activity against β-d-glucosides, and its identical freezing-sensitive phenotype confirmed that the SFR2 gene product is essential for freezing tolerance.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
High resolution two-dimensional electrophoresis of proteins.
TL;DR: This technique provides a method for estimation of the number of proteins made by any biological system and can resolve proteins differing in a single charge and consequently can be used in the analysis of in vivo modifications resulting in a change in charge.
Book
Methods of Enzymatic Analysis
TL;DR: Methods of enzymatic analysis, Methods of enzymes analysis, the authors, Methods of enzyme analysis, enzymatics, methods of enzymes, and methods of analysis, method of enzymes.
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