Journal ArticleDOI
Plant growth-promoting Pseudomonas putida WCS358 produces and secretes four cyclic dipeptides: cross-talk with quorum sensing bacterial sensors.
TLDR
Surprisingly, it was observed that the most abundant compounds in these fractions were cyclic dipeptides (diketopiperazines, DKPs), a rather novel finding in Gram-negative bacteria.Abstract:
The most universal cell-cell signaling mechanism in Gram-negative bacteria occurs via the production and response to a class of small diffusible molecules called N-acylhomoserine lactones (AHLs). This communication is called quorum sensing and is responsible for the regulation of several physiological processes and many virulence factors in pathogenic bacteria. The detection of these molecules has been rendered possible by the utilization of genetically engineered bacterial biosensors which respond to the presence of exogenously supplied AHLs. In this study, using diverse bacterial biosensors, several biosensor activating fractions were purified by organic extraction, HPLC and TLC of cell-free culture supernatants of plant growth-promoting Pseudomonas putida WCS358. Surprisingly, it was observed that the most abundant compounds in these fractions were cyclic dipeptides (diketopiperazines, DKPs), a rather novel finding in Gram-negative bacteria. The purification, characterization, chemical synthesis of four DKPs are reported and their possible role in cell-cell signaling is discussed.read more
Citations
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Journal ArticleDOI
Intracellular and extracellular PGPR: commonalities and distinctions in the plant–bacterium signaling processes
E.J. Gray,Donald L. Smith +1 more
TL;DR: A detailed review of the signaling process of rhizobia (iPGPR), including plant-to-bacteria signal molecules, followed by bacterial perception and consequent production of bacteria- to-plant signals, is provided.
Journal ArticleDOI
2,5-Diketopiperazines: synthesis, reactions, medicinal chemistry, and bioactive natural products.
Journal ArticleDOI
Exploiting Quorum Sensing To Confuse Bacterial Pathogens
Breah LaSarre,Michael J. Federle +1 more
TL;DR: Overall, the evidence suggests that QSI therapy may be efficacious against some, but not necessarily all, bacterial pathogens, and several failures and ongoing concerns that may steer future studies in productive directions are discussed.
Journal ArticleDOI
Quorum sensing in Gram-negative bacteria: small-molecule modulation of AHL and AI-2 quorum sensing pathways.
TL;DR: This work has shown that quorum sensing enhances the survival prospects of the bacteria because a coordinated attack on the host is only made when the bacterial population reaches a high population density, increasing the likelihood that the hosts defenses will be successfully overwhelmed.
Journal ArticleDOI
Does efficiency sensing unify diffusion and quorum sensing
Burkhard A. Hense,Christina Kuttler,Johannes Müller,Michael Rothballer,Anton Hartmann,Jan-Ulrich Kreft +5 more
TL;DR: It is argued that these conflicting concepts can be unified by a new hypothesis, efficiency sensing, and that some of the problems associated with signalling in complex environments, as well as the problem of maintaining honesty in signalling, can be avoided when the signalling cells grow in microcolonies.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Quorum sensing and Chromobacterium violaceum: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones
Kay H. McClean,Michael K. Winson,Leigh Fish,Adrian Taylor,Siri Ram Chhabra,Miguel Cámara,Mavis Daykin,John H. Lamb,Simon Swift,Barrie W. Bycroft,Gordon S. A. B. Stewart,Paul Williams +11 more
TL;DR: The ability of CV026 to respond to a series of synthetic AHL and N-acylhomocysteine thiolactone (AHT) analogues is explored, greatly extending the ability to detect a wide spectrum of AHL signal molecules.
Journal ArticleDOI
Census and consensus in bacterial ecosystems: the LuxR-LuxI family of quorum-sensing transcriptional regulators.
TL;DR: Genetic analyses of particular bacterial genes, operons, or regulons that are expressed preferentially at high cell densities have revealed a high degree of functional conservation, while also uncovering features that are unique to each.
Journal ArticleDOI
Quorum sensing by peptide pheromones and two-component signal-transduction systems in Gram-positive bacteria
TL;DR: Cell‐density‐dependent gene expression appears to be widely spread in bacteria, and genetic linkage of the common elements involved results in autoregulation of peptide‐pheromone production.
Journal ArticleDOI
Detecting and characterizing N-acyl-homoserine lactone signal molecules by thin-layer chromatography
Paul D. Shaw,Gao Ping,Sean L. Daly,Chung Cha,John E. Cronan,Kenneth L. Rinehart,Stephen K. Farrand +6 more
TL;DR: The assay can be used to screen cultures of bacteria for acyl-homoserine lactones, for quantifying the amounts of these molecules produced, and as an analytical and preparative aid in determining the structures of these signal molecules.