Quorum-sensing cross talk: isolation and chemical characterization of cyclic dipeptides from Pseudomonas aeruginosa and other gram-negative bacteria.
Matthew T. G. Holden,Siri Ram Chhabra,Rocky de Nys,Paul Stead,Nigel J. Bainton,Philip J. Hill,Mike Manefield,Naresh Kumar,Maurice Labatte,Dacre England,Scott A. Rice,Michael Givskov,George P. C. Salmond,Gordon S. A. B. Stewart,Barrie W. Bycroft,Staffan Kjelleberg,Paul Williams +16 more
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In this article, the diketopiperazines (DKPs) were found to activate the N-acylhomoserine lactone (AHL) biosensor in a concentration-dependent manner.Abstract:
In cell-free Pseudomonas aeruginosa culture supernatants, we identified two compounds capable of activating an N-acylhomoserine lactone (AHL) biosensor. Mass spectrometry and NMR spectroscopy revealed that these compounds were not AHLs but the diketopiperazines (DKPs), cyclo(ΔAla-l-Val) and cyclo(l-Pro-l-Tyr) respectively. These compounds were also found in cell-free supernatants from Proteus mirabilis, Citrobacter freundii and Enterobacter agglomerans [cyclo(ΔAla-l-Val) only]. Although both DKPs were absent from Pseudomonas fluorescens and Pseudomonas alcaligenes, we isolated, from both pseudomonads, a third DKP, which was chemically characterized as cyclo(l-Phe-l-Pro). Dose–response curves using a LuxR-based AHL biosensor indicated that cyclo(ΔAla-l-Val), cyclo(l-Pro-l-Tyr) and cyclo(l-Phe-l-Pro) activate the biosensor in a concentration-dependent manner, albeit at much higher concentrations than the natural activator N-(3-oxohexanoyl)-l-homoserine lactone (3-oxo-C6-HSL). Competition studies showed that cyclo(ΔAla-l-Val), cyclo(l-Pro-l-Tyr) and cyclo(l-Phe-l-Pro) antagonize the 3-oxo-C6-HSL-mediated induction of bioluminescence, suggesting that these DKPs may compete for the same LuxR-binding site. Similarly, DKPs were found to be capable of activating or antagonizing other LuxR-based quorum-sensing systems, such as the N-butanoylhomoserine lactone-dependent swarming motility of Serratia liquefaciens. Although the physiological role of these DKPs has yet to be established, their activity suggests the existence of cross talk among bacterial signalling systems.read more
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The Role of Root Exudates in Rhizosphere Interactions with Plants and Other Organisms
TL;DR: Recent advances in elucidating the role of root exudates in interactions between plant roots and other plants, microbes, and nematodes present in the rhizosphere are described.
Journal ArticleDOI
QUORUM SENSING: Cell-to-Cell Communication in Bacteria
TL;DR: This review focuses on the architectures of bacterial chemical communication networks; how chemical information is integrated, processed, and transduced to control gene expression; how intra- and interspecies cell-cell communication is accomplished; and the intriguing possibility of prokaryote-eukaryote cross-communication.
Journal ArticleDOI
Biofilms as complex differentiated communities.
TL;DR: It is submitted that complex cell-cell interactions within prokaryotic communities are an ancient characteristic, the development of which was facilitated by the localization of cells at surfaces, which may have provided the protective niche in which attached cells could create a localized homeostatic environment.
Journal ArticleDOI
Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture
TL;DR: The progress to date in using the rhizosphere bacteria in a variety of applications related to agricultural improvement along with their mechanism of action with special reference to plant growth-promoting traits are summarized and discussed in this review.
Journal ArticleDOI
Quorum‐sensing in Gram‐negative bacteria
Neil A. Whitehead,Anne M. L. Barnard,Holly Slater,Natalie J. L. Simpson,George P. C. Salmond +4 more
TL;DR: The current state of research concerning acyl H SL-mediated quorum-sensing is reviewed and two non-acyl HSL-based systems utilised by the phytopathogens Ralstonia solanacearum and Xanthomonas campestris are described.
References
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Quorum sensing and Chromobacterium violaceum: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones
Kay H. McClean,Michael K. Winson,Leigh Fish,Adrian Taylor,Siri Ram Chhabra,Miguel Cámara,Mavis Daykin,John H. Lamb,Simon Swift,Barrie W. Bycroft,Gordon S. A. B. Stewart,Paul Williams +11 more
TL;DR: The ability of CV026 to respond to a series of synthetic AHL and N-acylhomocysteine thiolactone (AHT) analogues is explored, greatly extending the ability to detect a wide spectrum of AHL signal molecules.
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Common virulence factors for bacterial pathogenicity in plants and animals
Laurence G. Rahme,Emily J. Stevens,Sean F. Wolfort,Jing Shao,Ronald G. Tompkins,Frederick M. Ausubel +5 more
TL;DR: A Pseudomonas aeruginosa strain (UCBPP-PA14) is infectious both in an Arabidopsis thaliana leaf infiltration model and in a mouse full-thickness skin burn model, indicating that these genes encode virulence factors required for the full expression of pathogenicity in both plants and animals.
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Census and consensus in bacterial ecosystems: the LuxR-LuxI family of quorum-sensing transcriptional regulators.
TL;DR: Genetic analyses of particular bacterial genes, operons, or regulons that are expressed preferentially at high cell densities have revealed a high degree of functional conservation, while also uncovering features that are unique to each.
Journal ArticleDOI
Structural identification of autoinducer of Photobacterium fischeri luciferase.
Anatol Eberhard,Alma L. Burlingame,C. Eberhard,George L. Kenyon,Kenneth H. Nealson,Norman J. Oppenheimer +5 more
TL;DR: In this paper, an autoinducer excreted by Photobacterium fischeri strain MJ-1 was isolated from the cell-free medium by extraction with ethyl acetate, evaporation of solvent, workup with ethanol-water mixtures, and silica gel chromatography.