Point-of-care nucleic acid testing for infectious diseases
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TLDR
Recent technical advances that might be able to address limitations in nucleic acid amplification and detection in a cost-effective, robust, and user-friendly format are described.About:
This article is published in Trends in Biotechnology.The article was published on 2011-05-01 and is currently open access. It has received 752 citations till now. The article focuses on the topics: Nucleic acid amplification technique.read more
Citations
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Journal ArticleDOI
Point of care diagnostics: Status and future
TL;DR: This chapter discusses the development of personalized medicine and home testing in the developing world, and some of the strategies used to achieve this goal have not yet been developed.
Journal ArticleDOI
SHERLOCK: nucleic acid detection with CRISPR nucleases.
Max J. Kellner,Jeremy G. Koob,Jonathan S. Gootenberg,Jonathan S. Gootenberg,Jonathan S. Gootenberg,Omar O. Abudayyeh,Omar O. Abudayyeh,Omar O. Abudayyeh,Feng Zhang +8 more
TL;DR: Step-by-step instructions for setting up SHERLOCK assays with recombinase-mediated polymerase pre-amplification of DNA or RNA and subsequent Cas13- or Cas12-mediated detection via fluorescence and colorimetric readouts that provide results in <1 h with a setup time of less than 15 min are provided.
Journal ArticleDOI
Diagnostic point-of-care tests in resource-limited settings
Paul K. Drain,Emily P. Hyle,Farzad Noubary,Farzad Noubary,Farzad Noubary,Kenneth A. Freedberg,Douglas Wilson,William R. Bishai,William Rodriguez,Ingrid V. Bassett +9 more
TL;DR: A framework for the assessment of point-of-care tests is proposed, and the term test efficacy is suggested to describe the ability of a diagnostic test to support a clinical decision within its operational context, and revised criteria for an ideal diagnostic point- of-care test in resource-limited settings are proposed.
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Point-of-care testing for infectious diseases: diversity, complexity, and barriers in low- and middle-income countries.
Nitika Pant Pai,Caroline Vadnais,Claudia M. Denkinger,Claudia M. Denkinger,Nora Engel,Madhukar Pai,Madhukar Pai +6 more
TL;DR: A framework for envisioning how point-of-care testing can be applied to infectious diseases in low- and middle-income countries is discussed.
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Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes.
TL;DR: The colorimetric detection of amplification presented here represents a generally applicable approach for visual detection of nucleic acid amplification, enabling molecular diagnostic tests to be analyzed immediately without the need for specialized and expensive instrumentation.
References
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Dawn M. Sievert,Philip Ricks,Jonathan R. Edwards,Amy Schneider,Jean B. Patel,Arjun Srinivasan,Alex Kallen,Brandi Limbago,Scott K. Fridkin +8 more
TL;DR: The frequency of selected antimicrobial resistance patterns among pathogens causing device-associated and procedure-associated healthcare-associated infections reported by hospitals in the National Healthcare Safety Network (NHSN) is described.
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Catharina Boehme,Pamela Nabeta,Doris Hillemann,Mark P. Nicol,Shubhada Shenai,Fiorella Krapp,Jenny Allen,Rasim Tahirli,Robert Blakemore,Roxana Rustomjee,Ana Milovic,Martin Jones,Sean M. O'Brien,David H. Persing,Sabine Ruesch-Gerdes,Eduardo Gotuzzo,Camilla Rodrigues,David Alland,Mark D. Perkins +18 more
TL;DR: The MTB/RIF test provided sensitive detection of tuberculosis and rifampin resistance directly from untreated sputum in less than 2 hours with minimal hands-on time.
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DNA Detection Using Recombination Proteins
TL;DR: RPA couples isothermal recombinase polymerase-driven primer targeting of template material with strand-displacement DNA synthesis and achieves exponential amplification with no need for pretreatment of sample DNA, thereby establishing an instrument-free DNA testing system.
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An integrated nanoliter DNA analysis device
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TL;DR: A device was developed that uses microfabricated fluidic channels, heaters, temperature sensors, and fluorescence detectors to analyze nanoliter-size DNA samples to facilitate the use of DNA analysis in applications such as rapid medical diagnostics and point-of-use agricultural testing.
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Early Antiretroviral Therapy and Mortality among HIV-Infected Infants
Avy Violari,Mark F. Cotton,Diana M. Gibb,Abdel Babiker,Jan Steyn,Shabir A. Madhi,Patrick Jean-Philippe,James McIntyre +7 more
TL;DR: Early HIV diagnosis and early antiretroviral therapy reduced early infant mortality by 76% and HIV progression by 75%.