Primary Structure Effects on Peptide Group Hydrogen Exchange
TLDR
The results provide the information necessary to evaluate measured protein NH to ND exchange rates by comparing them with rates to be expected for the same amino acid sequence is unstructured aligo‐ and polypeptides.Abstract:
The rate of exchange of peptide group NH hydrogens with the hydrogens of aqueous solvent is sensitive to neighboring side chains. To evaluate the effects of protein side chains, all 20 naturally occurring amino acids were studied using dipeptide models. Both inductive and steric blocking effects are apparent. The additivity of nearest-neighbor blocking and inductive effects was tested in oligo- and polypeptides and, surprisingly, confirmed. Reference rates for alanine-containing peptides were determined and effects of temperature considered. These results provide the information necessary to evaluate measured protein NH to ND exchange rates by comparing them with rates to be expected for the same amino acid sequence is unstructured oligo- and polypeptides. The application of this approach to protein studies is discussed.read more
Citations
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Protein dynamics and function from solution state NMR spectroscopy.
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References
More filters
Journal ArticleDOI
MLEV-17-based two-dimensional homonuclear magnetization transfer spectroscopy
Ad Bax,Donald G. Davis +1 more
TL;DR: In this article, a new mixing scheme based on the MLEV-16 composite pulse decoupling cycle (II) was proposed, which is less sensitive to pulse imperfections and provides net magnetization transfer over a substantial bandwidth with only limited rf power.
Journal ArticleDOI
Coherence transfer by isotropic mixing: Application to proton correlation spectroscopy
Journal ArticleDOI
Use of glass electrodes to measure acidities in deuterium oxide1,2
Paul K. Glasoe,F. A. Long +1 more
TL;DR: In this article, a pH meter reading in D/sub 2/O solutions was 0-40 pH unit lower than in H/sub O solutions, attributed to the glass electrode.
Journal ArticleDOI
Dithiothreitol, a New Protective Reagent for SH Groups*
TL;DR: Strominger, J. L. H. (1960), Instruction Manual and Handbook, Beckman/Spinco Model 120 Amino Acid Analyzer, Palo Alto, California,Beckman Instruments Inc., Spinco Division.
Journal ArticleDOI
A two-dimensional nuclear overhauser enhancement (2d noe) experiment for the elucidation of complete proton-proton cross-relaxation networks in biological macromolecules
TL;DR: The 2D NOE experiment has the principal advantage that it avoids detrimental effects arising from the limited selectivity of preirradiation in crowded spectral regions, and yields with a single instrument setting a complete network of NOE's between all the protons in the macromolecule.
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