Journal ArticleDOI
Probing conformational changes in proteins by mass spectrometry
TLDR
The authors describe the first use of mass spectrometry for probing conformational changes in proteins in a manner analogous to that employed in techniques like optical rotary dispersion, circular dichroism, and spectrophotometry.Abstract:
Mass spectrometry has found wide application for the elucidation of the primary structures of proteins. However, with the exception of topographical studies of membrane-bound proteins, mass spectrometry has not previously been utilized to obtain information concerning in three-dimensional conformation of proteins. In the present communication, the authors describe the first use of mass spectrometry for probing conformational changes in proteins in a manner analogous to that employed in techniques like optical rotary dispersion, circular dichroism, and spectrophotometry.read more
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Higher order structure in the gas phase reflects solution structure
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Revealing Higher Order Protein Structure Using Mass Spectrometry
TL;DR: A personal perspective of the increasingly useful role that mass spectrometric techniques are exerting during the elucidation of higher order protein structures and a vision for the future role of MS-based techniques in the development of a multi-scale molecular microscope is provided.
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Native Mass Spectrometry in Fragment-Based Drug Discovery.
Liliana Pedro,Ronald J. Quinn +1 more
TL;DR: Key attributes of native MS for FBDD campaigns are highlighted, including the capability to directly detect weak ligand binding to proteins, to determine stoichiometry, relative or absolute binding affinities and specificities.
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The Role of Fluorinated Alcohols as Mobile Phase Modifiers for LC-MS Analysis of Oligonucleotides
TL;DR: It is found that more acidic fluorinated alcohols assist with the transfer of oligonucleotides with secondary structure (e.g., folded strands and hairpins) into the gas phase.
Journal ArticleDOI
High-accuracy mass measurement as a tool for studying proteins.
Rong Wang,Brian T. Chait +1 more
TL;DR: Electrospray ionization and matrix-assisted laser desorption/ionization, two new mass spectroscopy methods for the accurate measurement of molecular masses of individual peptide and protein molecules, are finding great utility for the solution of problems in biological research.
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